Evidence map›Paper›PMID 25962973›Full record

ArticleMolecular imaging and biology2015

Demonstration of Tightly Radiation-Controlled Molecular Switch Based on CArG Repeats by In Vivo Molecular Imaging.

Ya-Ju Hsieh, Luen Hwu, Chien-Chih Ke, Ai-Lin Huang, Fu-Du Chen, Shyh-Jong Wu, Sharon Chia-Ju Chen, Yong-Hua Zhao, Ren-Shyan Liu

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Article in Molecular imaging and biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Ya-Ju HsiehDepartment of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Luen HwuMolecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei, Taiwan.
Chien-Chih KeBiomedical Imaging Research Center, National Yang-Ming University, Taipei, Taiwan.
Ai-Lin HuangDepartment of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
Fu-Du ChenBiomedical Imaging Research Center, National Yang-Ming University, Taipei, Taiwan.
Shyh-Jong WuDepartment of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Sharon Chia-Ju ChenDepartment of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Yong-Hua ZhaoFaculty of Chinese Medicine, Macau University of Science and Technology, Macau, China.
Ren-Shyan LiuMolecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei, Taiwan. rsliu@vghtpe.gov.tw.
Kaohsiung Medical University · TWNational Yang Ming Chiao Tung University · TWNational Yang Ming Chiao Tung University · TWMacau University of Science and Technology · MONational Yang Ming University Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposePromoters developed for radiogene therapy always show non-negligible transcriptional activities, even when cells are not irradiated. This study developed a tightly radiation-controlled molecular switch based on radiation responsive element (CArG) repeats for in vivo molecular imaging using the Cre/loxP system. PROCEDURES: Different numbers of CArG repeats were cloned as a basal promoter directly, and its pre- and postirradiation transcriptional activities were analyzed by luciferase assay. Nine CArG repeats (E9) were chosen for use as a radiation-controlled molecular switch for the Cre/loxP system, and the feasibility of the switch in vitro and in vivo was demonstrated by luciferase assay and bioluminescence imaging, respectively.

resultsThe E9 promoter, which exhibits extremely low transcriptional activity, showed a 1.8-fold enhancement after irradiation with a clinical dose of 2 Gy. Both in vitro and in vivo results indicated that E9 is relatively inert but sufficient to trigger the Cre/loxP system. The luciferase activity of stable H1299/pSTOP-FLuc cells transfected with pE9-NLSCre and exposed to 2-Gy radiation can reach 44 % of that of the same cells transfected with pCMV-NLSCre and not subjected to irradiation. By contrast, no appreciable difference was observed in reporter gene expression in both H1299/pSTOPFluc cells and tumors transfected with pE4Pcmv-NLSCre before and after irradiation, because the strong basal transcriptional activity of the CMV promoter, which acts as a copartner of E4, masked the response of E4 to radiation.

conclusionsOur results provide detailed insight into CArG elements as a radiation-controlled molecular switch that can facilitate the development of radiogene therapy.

Indexed as

Molecular ImagingRepetitive Sequences, Nucleic AcidAnimalsCell Line, TumorFemaleHumansIntegrasesMiceMice, Inbred NODMice, SCIDPlasmidsPromoter Regions, GeneticTranscription, GeneticCre recombinaseIntegrasesCre/loxp systemMicropositron emission tomographyMolecular switchRadiation-responsive elements

Identifiers

PMID25962973
OpenAlexW327290472

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.