Evidence map›Paper›PMID 25962786›Full record

ArticleNature communications2015

Anammox Planctomycetes have a peptidoglycan cell wall.

Muriel C F van Teeseling, Rob J Mesman, Erkin Kuru, Akbar Espaillat, Felipe Cava, Yves V Brun, Michael S VanNieuwenhze, Boran Kartal, Laura van Niftrik

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
88citing papers in PubMed, 1 pooled it
11.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

88 citing papers in PubMed, 1 synthesis or guideline pooled it, 254 citations in OpenAlex.

  1. Pooled it
  2. Life (Basel, Switzerland) · 2026
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  19. MAGs-centric crack: how long will, spore-positiveFrontiers in microbiology · 2024
    Article
  20. Article

28 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 4 countries.

Muriel C F van TeeselingDepartment of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen 6525AJ, The Netherlands.
Rob J MesmanDepartment of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen 6525AJ, The Netherlands.
Erkin KuruInterdisciplinary Biochemistry Program, Indiana University, Bloomington, Indiana 47405, USA.
Akbar EspaillatDepartment of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, Umeå SE-90187, Sweden.
Felipe CavaDepartment of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, Umeå SE-90187, Sweden.ORCID 000000015995718X
Yves V BrunDepartment of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Michael S VanNieuwenhzeDepartment of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Boran Kartal1] Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen 6525AJ, The Netherlands [2] Department of Biochemistry and Microbiology, Laboratory of Microbiology, Gent University, Gent 9000, Belgium.
Laura van NiftrikDepartment of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen 6525AJ, The Netherlands.
Radboud University Nijmegen · NLIndiana University Bloomington · USUmeå University · SE

Funding

GLOBAL CONTROL OF DIFFERENTIATION IN CAULOBACTERR01GM051986 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI BRUN, YVES V · 1995 to 2016
$6.0M
Dynamics of bacterial peptidoglycan synthesisR01GM113172 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI BRUN, YVES V, VANNIEUWENHZE, MICHAEL S · 2015 to 2018
$3.5M
Acquisition of a DeltaVision OMX Super-Resolution Imaging SystemS10RR028697 · NCRR · INDIANA UNIVERSITY INDIANAPOLIS · PI WALCZAK, CLAIRE E · 2010 to 2010
$1.2M
European Research Council 232937NCRR NIH HHS S10 RR028697NCRR NIH HHS S10RR028697NIGMS NIH HHS GM051986NIGMS NIH HHS R01 GM051986NIGMS NIH HHS R01 GM113172
6 · The paper itself

Abstract

Planctomycetes are intriguing microorganisms that apparently lack peptidoglycan, a structure that controls the shape and integrity of almost all bacterial cells. Therefore, the planctomycetal cell envelope is considered exceptional and their cell plan uniquely compartmentalized. Anaerobic ammonium-oxidizing (anammox) Planctomycetes play a key role in the global nitrogen cycle by releasing fixed nitrogen back to the atmosphere as N2. Here using a complementary array of state-of-the-art techniques including continuous culturing, cryo-transmission electron microscopy, peptidoglycan-specific probes and muropeptide analysis, we show that the anammox bacterium Kuenenia stuttgartiensis contains peptidoglycan. On the basis of the thickness, composition and location of peptidoglycan in K. stuttgartiensis, we propose to redefine Planctomycetes as Gram-negative bacteria. Our results demonstrate that Planctomycetes are not an exception to the universal presence of peptidoglycan in bacteria.

Indexed as

Ammonium CompoundsAnaerobiosisCell WallOxidation-ReductionPeptidoglycanPlanctomycetalesAmmonium CompoundsPeptidoglycan

Identifiers

PMID25962786
PMCPMC4432595
OpenAlexW767528777

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.