Evidence map›Paper›PMID 25962772›Full record

ArticleThe American journal of tropical medicine and hygiene2015

Comparison of Fluorescence Microscopy and Different Growth Media Culture Methods for Acanthamoeba Keratitis Diagnosis.

Avi Peretz, Yuval Geffen, Soergiu D Socea, Nina Pastukh, Shmuel Graffi

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. American Academy of Optometry Microbial Keratitis Think Tank.Optometry and vision science : official publication of the American Academy of Optometry · 2021
    Article
  3. Growth comparison ofHeliyon · 2020
    Article
  4. African journal of infectious diseases · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Avi PeretzClinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel aperetz@poria.health.gov.il.
Yuval GeffenClinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
Soergiu D SoceaClinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
Nina PastukhClinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
Shmuel GraffiClinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
Poriya Medical Center · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acanthamoeba keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying Acanthamoeba. In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media-non-nutrient agar (NNA), culture on liquid growth media-peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.

Indexed as

AcanthamoebaAcanthamoeba KeratitisAdultCorneaCulture MediaCulture TechniquesFemaleHumansMaleMicroscopy, FluorescenceSpecimen HandlingCulture Media

Identifiers

PMID25962772
PMCPMC4530754
OpenAlexW2406596908

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.