Evidence map›Paper›PMID 25962137›Full record

ArticlePloS one2015

eEF-2 Phosphorylation Down-Regulates P-Glycoprotein Over-Expression in Rat Brain Microvessel Endothelial Cells.

Xing Hua Tang, Xun Yi Wu, Lan Xu, You Xin Fang, Ping Wang, Guo Xing Zhu, Zhen Hong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Xing Hua TangDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Xun Yi WuDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Lan XuDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
You Xin FangDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Ping WangDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Guo Xing ZhuDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Zhen HongDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Huashan Hospital · CNFudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveWe investigated whether glutamate, NMDA receptors, and eukaryote elongation factor-2 kinase (eEF-2K)/eEF-2 regulate P-glycoprotein expression, and the effects of the eEF-2K inhibitor NH125 on the expression of P-glycoprotein in rat brain microvessel endothelial cells (RBMECs).

methodsCortex was obtained from newborn Wistar rat brains. After surface vessels and meninges were removed, the pellet containing microvessels was resuspended and incubated at 37°C in culture medium. Cell viability was assessed by the MTT assay. RBMECs were identified by immunohistochemistry with anti-vWF. P-glycoprotein, phospho-eEF-2, and eEF-2 expression were determined by western blot analysis. Mdr1a gene expression was analyzed by RT-PCR.

resultsMdr1a mRNA, P-glycoprotein and phospho-eEF-2 expression increased in L-glutamate stimulated RBMECs. P-glycoprotein and phospho-eEF-2 expression were down-regulated after NH125 treatment in L-glutamate stimulated RBMECs.

conclusionseEF-2K/eEF-2 should have played an important role in the regulation of P-glycoprotein expression in RBMECs. eEF-2K inhibitor NH125 could serve as an efficacious anti-multidrug resistant agent.

Indexed as

Down-RegulationAnimalsATP Binding Cassette Transporter, Subfamily B, Member 1BrainCells, CulturedElongation Factor 2 KinaseEndothelial CellsEndothelium, VascularMicrovesselsPhosphorylationRatsRats, WistarATP Binding Cassette Transporter, Subfamily B, Member 1Elongation Factor 2 Kinase

Identifiers

PMID25962137
PMCPMC4427111
OpenAlexW227632404

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.