ArticleBioMed research international2015
Transcriptomic Analysis of mRNAs in Human Monocytic Cells Expressing the HIV-1 Nef Protein and Their Exosomes.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Using common features of viruses and EVs for a novel EV-based lateral flow test for HIV.Extracellular vesicle · 2025Article
- HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors.Molecular & cellular proteomics : MCP · 2023Article
- Review
- Immune cells-derived exosomes function as a double-edged sword: role in disease progression and their therapeutic applications.Biomarker research · 2022Review
- Soluble Insulin Receptor Levels in Plasma, Exosomes, and Urine and Its Association With HIV-Associated Neurocognitive Disorders.Frontiers in neurology · 2022Article
- HIV-1Biology direct · 2021Article
- miR-125b-5p inhibits cell proliferation by targeting ASCT2 and regulating the PI3K/AKT/mTOR pathway in an LPS-induced intestinal mucosa cell injury model.Experimental and therapeutic medicine · 2021Article
- Identification of specific microRNA-messenger RNA regulation pairs in four subtypes of breast cancer.IET systems biology · 2020Article
- MicroRNAs and exosomes: key players in HIV pathogenesis.HIV medicine · 2020Review
- How HIV Nef Proteins Hijack Membrane Traffic To Promote Infection.Journal of virology · 2019Review
- Multi-Objective Optimized Fuzzy Clustering for Detecting Cell Clusters from Single-Cell Expression Profiles.Genes · 2019Article
- Nef-induced differential gene expression in primary CD4+ T cells following infection with HIV-1 isolates.Virus genes · 2019Article
- Delivery of microRNAs by Extracellular Vesicles in Viral Infections: Could the News be Packaged?Cells · 2019Review
- Identification of gene signatures from RNA-seq data using Pareto-optimal cluster algorithm.BMC systems biology · 2018Article
- Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy.Scientific reports · 2018Article
- Detection of HIV-1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients.Advances in virology · 2018Article
- Exosomes in Pathogen Infections: A Bridge to Deliver Molecules and Link Functions.Frontiers in immunology · 2018Review
- Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes.Journal of neurovirology · 2017Article
- HIV-1 Nef Signaling in Intestinal Mucosa Epithelium Suggests the Existence of an Active Inter-kingdom Crosstalk Mediated by Exosomes.Frontiers in microbiology · 2017Review
- HIV-1 Nef promotes migration and chemokine synthesis of human basophils and mast cells through the interaction with CXCR4.Clinical and molecular allergy : CMA · 2016Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Nef protein of human immunodeficiency virus (HIV) promotes viral replication and progression to AIDS. Besides its well-studied effects on intracellular signaling, Nef also functions through its secretion in exosomes, which are nanovesicles containing proteins, microRNAs, and mRNAs and are important for intercellular communication. Nef expression enhances exosome secretion and these exosomes can enter uninfected CD4 T cells leading to apoptotic death. We have recently reported the first miRNome analysis of exosomes secreted from Nef-expressing U937monocytic cells. Here we show genome-wide transcriptome analysis of Nef-expressing U937 cells and their exosomes. We identified four key mRNAs preferentially retained in Nef-expressing cells; these code for MECP2, HMOX1, AARSD1, and ATF2 and are important for chromatin modification and gene expression. Interestingly, their target miRNAs are exported out in exosomes. We also identified three key mRNAs selectively secreted in exosomes from Nef-expressing U937 cells and their corresponding miRNAs being preferentially retained in cells. These are AATK, SLC27A1, and CDKAL and are important in apoptosis and fatty acid transport. Thus, our study identifies selectively expressed mRNAs in Nef-expressing U937 cells and their exosomes and supports a new mode on intercellular regulation by the HIV-1 Nef protein.
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Registered trials
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