ArticleBiotechnology for biofuels2015
Phaeodactylum tricornutum photorespiration takes part in glycerol metabolism and is important for nitrogen-limited response.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- The mosaic of aquatic photorespiration: evolution, diversity, and global biogeochemical impacts.The New phytologist · 2026Review
- Production and encapsulation of eicosapentaenoic acid (EPA)‑rich lipid extract from Phaeodactylum tricornutum under different cultivation conditions.World journal of microbiology & biotechnology · 2026Article
- Functional characterization of lactate metabolism and its key regulatory genes reveals lactate-mediated modulation of carbon and nitrogen metabolism in Phaeodactylum tricornutum.Microbial cell factories · 2025Article
- Phytohormone combined with nitrogen stress promoted carbon conversion in COFrontiers in bioengineering and biotechnology · 2025Article
- Metabolic response to a heterologous poly-3-hydroxybutyrate (PHB) pathway in Phaeodactylum tricornutum.Applied microbiology and biotechnology · 2024Article
- A Review of Fucoxanthin Biomanufacturing from Phaeodactylum tricornutum.Bioprocess and biosystems engineering · 2024Review
- Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid.The Plant cell · 2024Article
- Transcriptomic and metabolomic analyses revealed regulation mechanism of mixotrophic Cylindrotheca sp. glycerol utilization and biomass promotion.Biotechnology for biofuels and bioproducts · 2023Article
- Transcriptomic and metabolic signatures of diatom plasticity to light fluctuations.Plant physiology · 2022Article
- Metabolomic, proteomic and lactylated proteomic analyses indicate lactate plays important roles in maintaining energy and C:N homeostasis in Phaeodactylum tricornutum.Biotechnology for biofuels and bioproducts · 2022Article
- Effects of Taurine on Primary Metabolism and Transcription in a CoralFrontiers in microbiology · 2022Article
- Impact of organic carbon acquisition on growth and functional biomolecule production in diatoms.Microbial cell factories · 2021Review
- The Transition Toward Nitrogen Deprivation in Diatoms Requires Chloroplast Stand-By and Deep Metabolic Reshuffling.Frontiers in plant science · 2021Article
- Improving Fucoxanthin Production in Mixotrophic Culture of Marine DiatomFrontiers in bioengineering and biotechnology · 2020Article
- Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high COBMC biotechnology · 2019Article
- Investigating mixotrophic metabolism in the model diatomPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Light attenuates lipid accumulation while enhancing cell proliferation and starch synthesis in the glucose-fed oleaginous microalga Chlorella zofingiensis.Scientific reports · 2015Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroalgae are potential sources of biofuels and high-value compounds. Mixotrophic conditions usually promote growth of microalgae. The pennate diatom Phaeodactylum tricornutum, with its short life cycle, completely sequenced genome, and ease of transformation, can be used as a model for studying carbon metabolism in microalgae.
resultsWe compared the growth rate of P. tricornutum (IOCAS-001) under different conditions and labeled the cells using [(13)C]glycerol (GL). The results revealed GL promoted the growth of P. tricornutum. Ser and Gly were synthesized via photorespiration. The (13)C enrichment of Ser and Gly under nitrogen-limited conditions was much higher compared to other amino acids, indicating the enhancement of photorespiration. Addition of sodium acetate decreased the growth rate of P. tricornutum under nitrogen-limited conditions. Our results indicated that the GL carbon backbone enters the Calvin cycle in the form of dihydroxyacetone phosphate (DHAP), producing xylulose 5-phosphate (X5P) with a GL2_3-generated carbon backbone distributed at X5P1_2 and ribose 5-phosphate (R5P) with GL1-derived carbon atoms at R5P1 and R5P2. Both R5P and X5P can be converted into ribulose-1,5-bisphosphate (RuBP). By oxygenation of RuBP carboxylase/oxygenase (Rubisco) and metabolism through photorespiration, these RuBPs generate Ser and Gly with GL1 or GL2-derived carbon atoms at position 1 and GL1 or GL3-derived carbon atoms at other positions, resulting in a low level of (13)C enrichment of Gly1 and Ser1.
conclusionOur results indicated different strains of P. tricornutum have different mechanisms for organic carbon metabolism. Photorespiration is involved in GL metabolism and is important for the nitrogen-limited response in P. tricornutum. CLASSIFICATION: Metabolic flux analysis, microalgae.
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