ArticlePlant biotechnology journal2016
Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications.Biotechnology for biofuels and bioproducts · 2026Article
- Enhanced saccharification yields from rice straw by senescence-induced expression of a cytokinin biosynthesis gene in intragenic rice plants.Plant biotechnology (Tokyo, Japan) · 2026Article
- Glucuronoyl Esterase of Pathogenic Phanerochaete carnosa Induces Immune Responses in Aspen Independently of Its Enzymatic Activity.Plant biotechnology journal · 2026Article
- Wood-specific modification of glucuronoxylan can enhance growth in Populus.Journal of experimental botany · 2026Article
- Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification.Plant biotechnology journal · 2025Article
- Plant Cell Wall PolysaccharidePlants (Basel, Switzerland) · 2024Review
- Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility.Biotechnology for biofuels and bioproducts · 2024Article
- Integrity of xylan backbone affects plant responses to drought.Frontiers in plant science · 2024Article
- Overexpression of REDUCED WALL ACETYLATION C increases xylan acetylation and biomass recalcitrance in Populus.Plant physiology · 2023Article
- Xylobiose treatment triggers a defense-related response and alters cell wall composition.Plant molecular biology · 2023Article
- Fertilization of Microbial Composts: A Technology for Improving Stress Resilience in Plants.Plants (Basel, Switzerland) · 2023Review
- Plant Cell Wall Integrity Perturbations and Priming for Defense.Plants (Basel, Switzerland) · 2022Review
- Recombinant acetylxylan esterase of Halalkalibacterium halodurans NAH-Egypt: molecular and biochemical study.AMB Express · 2022Article
- Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency.Plant molecular biology · 2022Article
- Rational enzyme design for controlled functionalization of acetylated xylan for cell-free polymer biosynthesis.Carbohydrate polymers · 2021Article
- Article
- GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae.Plant cell reports · 2021Article
- Article
- Saccharification Potential of Transgenic Greenhouse- and Field-Grown Aspen Engineered for Reduced Xylan Acetylation.Frontiers in plant science · 2021Article
- Balanced Xylan Acetylation is the Key Regulator of Plant Growth and Development, and Cell Wall Structure and for Industrial Utilization.International journal of molecular sciences · 2020Review
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell wall hemicelluloses and pectins are O-acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing the extent of O-acetylation in xylan affects cell wall chemistry, plant performance and the recalcitrance of lignocellulose to saccharification. The Aspergillus niger acetyl xylan esterase AnAXE1 was expressed in Arabidopsis under the control of either the constitutively expressed 35S CAMV promoter or a woody-tissue-specific GT43B aspen promoter, and the protein was targeted to the apoplast by its native signal peptide, resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No significant alterations in cell wall composition were observed in the transgenic lines, but their xylans were more easily digested by a β-1,4-endoxylanase, and more readily extracted by hot water, acids or alkali. Enzymatic saccharification of lignocellulose after hot water and alkali pretreatments produced up to 20% more reducing sugars in several lines. Fermentation by Trametes versicolor of tissue hydrolysates from the line with a 30% reduction in acetyl content yielded ~70% more ethanol compared with wild type. Plants expressing 35S:AnAXE1 and pGT43B:AnAXE1 developed normally and showed increased resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis, probably due to constitutive activation of defence pathways. However, unintended changes in xyloglucan and pectin acetylation were only observed in 35S:AnAXE1-expressing plants. This study demonstrates that postsynthetic xylan deacetylation in woody tissues is a promising strategy for optimizing lignocellulosic biomass for biofuel production.
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