ArticleNature communications2015
Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Genomic instability and biofilm determinants inJournal of bacteriology · 2026Article
- Genomic instability and biofilm determinants inbioRxiv : the preprint server for biology · 2026Article
- CauloKO: an ordered transposon mutant library inJournal of bacteriology · 2026Article
- A genome-wide collection of barcoded single-gene deletion mutants in Salmonella enterica serovar Typhimurium.PloS one · 2024Article
- High-throughput functional genomics: A (myco)bacterial perspective.Molecular microbiology · 2023Review
- Best Practices in Designing, Sequencing, and Identifying Random DNA Barcodes.Journal of molecular evolution · 2023Review
- Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening.Genomics, proteomics & bioinformatics · 2022Article
- A systematic analysis of hypermucoviscosity and capsule reveals distinct and overlapping genes that impact Klebsiella pneumoniae fitness.PLoS pathogens · 2021Article
- Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense.mSystems · 2020Article
- Article
- Escherichia coli CFT073 Fitness Factors during Urinary Tract Infection: Identification Using an Ordered Transposon Library.Applied and environmental microbiology · 2020Article
- Global Assessment of Mycobacterium avium subsp.mSystems · 2019Article
- Higher-fitness yeast genotypes are less robust to deleterious mutations.Science (New York, N.Y.) · 2019Article
- Reference genome and comparative genome analysis for the WHO reference strain for Mycobacterium bovis BCG Danish, the present tuberculosis vaccine.BMC genomics · 2019Article
- Mycobacterial biomaterials and resources for researchers.Pathogens and disease · 2018Review
- Rapid curation of gene disruption collections using Knockout Sudoku.Nature protocols · 2017Article
- Defining Genetic Fitness Determinants and Creating Genomic Resources for an Oral Pathogen.Applied and environmental microbiology · 2017Article
- The coupon collector urn model with unequal probabilities in ecology and evolution.Journal of the Royal Society, Interface · 2017Review
- Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku.Nature communications · 2016Article
- High-throughput bacterial functional genomics in the sequencing era.Current opinion in microbiology · 2015Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse genetics research approaches require the availability of methods to rapidly generate specific mutants. Alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. However, this can be complex, expensive and time consuming. Here, we describe a robust, easy to implement parallel sequencing-based method (Cartesian Pooling-Coordinate Sequencing or CP-CSeq) that reports both on the identity as well as on the location of sequence-tagged biological entities in well-plate archived clone collections. We demonstrate this approach using a transposon insertion mutant library of the Mycobacterium bovis BCG vaccine strain, providing the largest resource of mutants in any strain of the M. tuberculosis complex. The method is applicable to any entity for which sequence-tagged identification is possible.
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