ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2015
Vitamins A and D have antagonistic effects on expression of effector cytokines and gut-homing integrin in human innate lymphoid cells.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 67 citations in OpenAlex.
- Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease.Cell reports. Medicine · 2026Article
- Regulation of innate lymphoid cell by microbial metabolites.Journal of molecular medicine (Berlin, Germany) · 2025Review
- Vitamin D and Chronic Rhinosinusitis with Nasal Polyps: A Narrative Review and Perspectives.Journal of clinical medicine · 2025Review
- Nutritional Support of Chronic Obstructive Pulmonary Disease.Nutrients · 2025Review
- The role of ILC2s in asthma combined with atopic dermatitis: bridging the gap from research to clinical practice.Frontiers in immunology · 2025Review
- Deciphering the Role of Innate Lymphoid Cells Group 3 in the Gut Microenvironment: A Narrative Review of Their Novel Contributions to Autoimmune Disease Pathogenesis.Journal of inflammation research · 2025Review
- Gender Differences in the Interplay between Vitamin D and Microbiota in Allergic and Autoimmune Diseases.Biomedicines · 2024Review
- Retinoic Acid Treatment Mitigates PM2.5-Induced Type 2 Inflammation: Insights into Modulation of Innate Immune Responses.International journal of molecular sciences · 2024Article
- Pet Wellness and Vitamin A: A Narrative Overview.Animals : an open access journal from MDPI · 2024Review
- Review
- Vitamin D and allergic diseases.Frontiers in immunology · 2024Review
- Is there a rationale for supplementing with vitamin D patients under treatment with allergen immunotherapy?Annals of medicine · 2023Article
- ILC3: a case of conflicted identity.Frontiers in immunology · 2023Review
- Modulation by luminal factors on the functions and migration of intestinal innate immunity.Frontiers in immunology · 2023Review
- Review
- Pollutants enhance IgE sensitization in the gut via local alteration of vitamin D-metabolizing enzymes.Mucosal immunology · 2022Article
- The Role of Crosstalk of Immune Cells in Pathogenesis of Chronic Spontaneous Urticaria.Frontiers in immunology · 2022Review
- Vitamin D Is Associated with α4β7+ Immunophenotypes and Predicts Vedolizumab Therapy Failure in Patients with Inflammatory Bowel Disease.Journal of Crohn's & colitis · 2021Article
- Resveratrol, EGCG and Vitamins Modulate Activated T Lymphocytes.Molecules (Basel, Switzerland) · 2021Article
- Mega doses of retinol: A possible immunomodulation in Covid-19 illness in resource-limited settings.Reviews in medical virology · 2021Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundRetinoic acid (RA), the main biologically active metabolite of vitamin A, is known to promote gut homing of lymphocytes, as well as various regulatory and effector immune responses. In contrast, the active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25D3), is predominantly immunosuppressive. Little is known about the direct effects of these vitamins on the recently identified innate lymphoid cells (ILCs).
objectiveWe sought to characterize the effects of RA and 1,25D3 on human ILCs.
methodsPeripheral blood mononuclear cells were isolated from 27 non-selected blood donor buffy coats, and ILCs were sorted by FACS. ILC1, ILC2, and ILC3 cells were cultured for 5 days with RA, 1,25D3, and various cytokines known to activate ILCs (IL-2, IL-7, IL-12, thymic stromal lymphopoietin (TSLP), IL-25, and IL-33). Cytokines produced by ILCs were measured in culture supernatants, and surface receptor expression was analysed by flow cytometry.
resultsRetinoic acid acted synergistically with IL-2 and other activating cytokines to induce expression of the gut-homing integrin α4β7 in ILCs, as well as production of IL-5 and IL-13 in ILC2 cells, and IFN-γ in ILC1 and ILC3 cells. Expression of integrin α4β7 and cytokine production in ILCs stimulated with RA + IL-2 was increased at least fourfold as compared to ILCs cultured with RA or IL-2 alone. In contrast, RA completely inhibited the IL-2-induced expression of cutaneous lymphocyte antigen (CLA) in ILCs. Moreover, addition of 1,25D3 to ILCs cultured with RA + IL-2 inhibited cytokine production and expression of integrin α4β7 by at least 30%.
conclusionsRetinoic acid and 1,25D3 have antagonistic effects on the expression of effector cytokines and gut-homing integrin by human ILCs. The balance between these vitamins may be an important factor in the functioning of ILCs and the diseases in which ILCs are implicated, such as allergic inflammation.
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