Evidence map›Paper›PMID 25959799›Full record

ArticleJournal of medical virology2015

Evaluation of a PCR/ESI-MS platform to identify respiratory viruses from nasopharyngeal aspirates.

Yong Lin, Yongfeng Fu, Menghua Xu, Liyun Su, Lingfeng Cao, Jin Xu, Xunjia Cheng

Open access · bronzeAbstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Mechanisms, Techniques and Devices of Airborne Virus Detection: A Review.International journal of environmental research and public health · 2023
    Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yong LinDepartment of Laboratory Medicine, Huashan Hospital of Fudan University, Shanghai, China.
Yongfeng FuDepartment of Medical Microbiology and Parasitology, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai, China.
Menghua XuDepartment of Clinical Laboratory Center, Children's Hospital of Fudan University, Shanghai, China.
Liyun SuDepartment of Clinical Laboratory Center, Children's Hospital of Fudan University, Shanghai, China.
Lingfeng CaoDepartment of Clinical Laboratory Center, Children's Hospital of Fudan University, Shanghai, China.
Jin XuDepartment of Clinical Laboratory Center, Children's Hospital of Fudan University, Shanghai, China.
Xunjia ChengDepartment of Medical Microbiology and Parasitology, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai, China.
Children's Hospital of Fudan University · CNShanghai Medical College of Fudan University · CNShanghai Hospital Development Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acute respiratory tract infection is a major cause of morbidity and mortality worldwide, particularly in infants and young children. High-throughput, accurate, broad-range tools for etiologic diagnosis are critical for effective epidemic control. In this study, the diagnostic capacities of an Ibis platform based on the PCR/ESI-MS assay were evaluated using clinical samples. Nasopharyngeal aspirates (NPAs) were collected from 120 children (<5 years old) who were hospitalized with lower respiratory tract infections between November 2010 and October 2011. The respiratory virus detection assay was performed using the PCR/ESI-MS assay and the DFA. The discordant PCR/ESI-MS and DFA results were resolved with RT-PCR plus sequencing. The overall agreement for PCR/ESI-MS and DFA was 98.3% (118/120). Compared with the results from DFA, the sensitivity and specificity of the PCR/ESI-MS assay were 100% and 97.5%, respectively. The PCR/ESI-MS assay also detected more multiple virus infections and revealed more detailed subtype information than DFA. Among the 12 original specimens with discordant results between PCR/ESI-MS and DFA, 11 had confirmed PCR/ESI-MS results. Thus, the PCR/ESI-MS assay is a high-throughput, sensitive, specific and promising method to detect and subtype conventional viruses in respiratory tract infections and allows rapid identification of mixed pathogens.

Indexed as

Child, PreschoolClinical Laboratory TechniquesFemaleFluorescent Antibody Technique, DirectHumansInfantMaleNasopharynxPolymerase Chain ReactionRespiratory Tract InfectionsSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationVirologyVirus DiseasesVirusesdiagnosisdirect immunofluorescent assay (DFA)electrospray ionization mass spectrometry (ESI/MS)respiratory tract infectionsvirus

Identifiers

PMID25959799
PMCPMC7166901
OpenAlexW1604677847

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.