ArticleParasites & vectors2015
Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Processing and mounting phlebotomine sand flies: a consensus guideline.Parasite (Paris, France) · 2026Article
- Appearance may be deceiving: Mexican sand flies (Diptera: Psychodidae: Phlebotominae) embrace a high diversity of cryptic species.Journal of insect science (Online) · 2025Article
- Age structure and parity status determination of Afrotropical malaria vectors using MALDI-TOF MS.Scientific reports · 2025Article
- Using deep learning artificial intelligence for sex identification and taxonomy of sand fly species.PloS one · 2025Article
- Making MALDI-TOF MS for entomological parameters accessible: A practical guide for in-house library creation.PloS one · 2025Article
- Article
- Species identification of phlebotomine sandflies using deep learning and wing interferential pattern (WIP).Scientific reports · 2023Article
- A Review of Historical Phlebotominae Taxonomy (Diptera: Psychodidae).Neotropical entomology · 2023Review
- Phylogenetic relations among Mexican phlebotomine sand flies (Diptera: Psychodidae) and their divergence time estimation.PloS one · 2023Article
- Wing Interferential Patterns (WIPs) and machine learning, a step toward automatized tsetse (Glossina spp.) identification.Scientific reports · 2022Article
- Applications of MALDI-TOF Mass Spectrometry to the Identification of Parasites and Arthropod Vectors of Human Diseases.Microorganisms · 2022Review
- MALDI-TOF MS Limits for the Identification of Mediterranean Sandflies of the SubgenusMicroorganisms · 2022Article
- MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.PLoS neglected tropical diseases · 2021Article
- New assessment of Anopheles vector species identification using MALDI-TOF MS.Malaria journal · 2021Article
- Sand flies (Diptera: Psychodidae) in eight Balkan countries: historical review and region-wide entomological survey.Parasites & vectors · 2020Review
- Evaluation of different storage times and preservation methods on phlebotomine sand fly DNA concentration and purity.Parasites & vectors · 2020Article
- Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library.PLoS neglected tropical diseases · 2019Article
- Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal.The American journal of tropical medicine and hygiene · 2019Article
- Improvement of mosquito identification by MALDI-TOF MS biotyping using protein signatures from two body parts.Parasites & vectors · 2018Article
- Understanding Leishmania parasites through proteomics and implications for the clinic.Expert review of proteomics · 2018Review
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRapid, accurate and high-throughput identification of vector arthropods is of paramount importance in surveillance programmes that are becoming more common due to the changing geographic occurrence and extent of many arthropod-borne diseases. Protein profiling by MALDI-TOF mass spectrometry fulfils these requirements for identification, and reference databases have recently been established for several vector taxa, mostly with specimens from laboratory colonies.
methodsWe established and validated a reference database containing 20 phlebotomine sand fly (Diptera: Psychodidae, Phlebotominae) species by using specimens from colonies or field-collections that had been stored for various periods of time.
resultsIdentical biomarker mass patterns ('superspectra') were obtained with colony- or field-derived specimens of the same species. In the validation study, high quality spectra (i.e. more than 30 evaluable masses) were obtained with all fresh insects from colonies, and with 55/59 insects deep-frozen (liquid nitrogen/-80 °C) for up to 25 years. In contrast, only 36/52 specimens stored in ethanol could be identified. This resulted in an overall sensitivity of 87 % (140/161); specificity was 100 %. Duration of storage impaired data counts in the high mass range, and thus cluster analyses of closely related specimens might reflect their storage conditions rather than phenotypic distinctness. A major drawback of MALDI-TOF MS is the restricted availability of in-house databases and the fact that mass spectrometers from 2 companies (Bruker, Shimadzu) are widely being used. We have analysed fingerprints of phlebotomine sand flies obtained by automatic routine procedure on a Bruker instrument by using our database and the software established on a Shimadzu system. The sensitivity with 312 specimens from 8 sand fly species from laboratory colonies when evaluating only high quality spectra was 98.3 %; the specificity was 100 %. The corresponding diagnostic values with 55 field-collected specimens from 4 species were 94.7 % and 97.4 %, respectively.
conclusionsA centralized high-quality database (created by expert taxonomists and experienced users of mass spectrometers) that is easily amenable to customer-oriented identification services is a highly desirable resource. As shown in the present work, spectra obtained from different specimens with different instruments can be analysed using a centralized database, which should be available in the near future via an online platform in a cost-efficient manner.
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