ArticleApplied and environmental microbiology2015
Energy Conservation Model Based on Genomic and Experimental Analyses of a Carbon Monoxide-Utilizing, Butyrate-Forming Acetogen, Eubacterium limosum KIST612.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 92 citations in OpenAlex.
- A CRISPR interference system for tunable gene expression integrated with a promoter library forMicrobiology spectrum · 2026Article
- Butyrate-associated antiproliferative and antitumor effects ofFrontiers in microbiology · 2026Article
- Implications of gut microbiota-mediated epigenetic modifications in intestinal diseases.Gut microbes · 2025Review
- Multi-omics analysis reveals the genetic basis for rapid COmSystems · 2025Article
- A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide.Nature communications · 2025Article
- Acetate Shock Loads Enhance CO Uptake Rates of Anaerobic Microbiomes.Microbial biotechnology · 2024Article
- Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts.FEMS microbiology ecology · 2024Article
- Redirecting electron flow in Acetobacterium woodii enables growth on CO and improves growth on formate.Nature communications · 2024Article
- Methanol as a co-substrate with COApplied microbiology and biotechnology · 2024Article
- The mechanisms, regulations, and functions of histone lysine crotonylation.Cell death discovery · 2024Review
- Sodium butyrate promotes gastrointestinal development of preweaning bull calves via inhibiting inflammation, balancing nutrient metabolism, and optimizing microbial community functions.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Review
- Formate-induced CO tolerance and methanogenesis inhibition in fermentation of syngas and plant biomass for carboxylate production.Biotechnology for biofuels and bioproducts · 2023Article
- Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Biological treatment of biowaste as an innovative source of CO-The role of composting process.Frontiers in bioengineering and biotechnology · 2023Review
- The extent of nitrogen isotopic fractionation in rumen bacteria is associated with changes in rumen nitrogen metabolism.PloS one · 2023Article
- Protein acylation: mechanisms, biological functions and therapeutic targets.Signal transduction and targeted therapy · 2022Review
- Biosynthesis of butyrate from methanol and carbon monoxide by recombinant Acetobacterium woodii.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- ATP synthesis in an ancient ATP synthase at low driving forces.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- High methanol-to-formate ratios induce butanol production in Eubacterium limosum.Microbial biotechnology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eubacterium limosum KIST612 is one of the few acetogens that can produce butyrate from carbon monoxide. We have used a genome-guided analysis to delineate the path of butyrate formation, the enzymes involved, and the potential coupling to ATP synthesis. Oxidation of CO is catalyzed by the acetyl-coenzyme A (CoA) synthase/CO dehydrogenase and coupled to the reduction of ferredoxin. Oxidation of reduced ferredoxin is catalyzed by the Rnf complex and Na(+) dependent. Consistent with the finding of a Na(+)-dependent Rnf complex is the presence of a conserved Na(+)-binding motif in the c subunit of the ATP synthase. Butyrate formation is from acetyl-CoA via acetoacetyl-CoA, hydroxybutyryl-CoA, crotonyl-CoA, and butyryl-CoA and is consistent with the finding of a gene cluster that encodes the enzymes for this pathway. The activity of the butyryl-CoA dehydrogenase was demonstrated. Reduction of crotonyl-CoA to butyryl-CoA with NADH as the reductant was coupled to reduction of ferredoxin. We postulate that the butyryl-CoA dehydrogenase uses flavin-based electron bifurcation to reduce ferredoxin, which is consistent with the finding of etfA and etfB genes next to it. The overall ATP yield was calculated and is significantly higher than the one obtained with H2 + CO2. The energetic benefit may be one reason that butyrate is formed only from CO but not from H2 + CO2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.