ArticleApplied and environmental microbiology2015
Use of Hybridization Chain Reaction-Fluorescent In Situ Hybridization To Track Gene Expression by Both Partners during Initiation of Symbiosis.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Single-cell detection and quantification of the microbiota by MicFLY.Cell host & microbe · 2026Article
- Single-cell quantification of the microbiota by flow cytometry: MicFLY.bioRxiv : the preprint server for biology · 2025Article
- A targeted approach to enrich host-associated bacteria for metagenomic sequencing.FEMS microbes · 2024Article
- Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology.Integrative and comparative biology · 2023Article
- Visualization of mRNA Expression in Pseudomonas aeruginosa Aggregates Reveals Spatial Patterns of Fermentative and Denitrifying Metabolism.Applied and environmental microbiology · 2022Article
- Advances and Challenges in FluorescenceFrontiers in microbiology · 2022Article
- Optimizing the hybridization chain reaction-fluorescence in situ hybridization (HCR-FISH) protocol for detection of microbes in sediments.Marine life science & technology · 2021Article
- A lasting symbiosis: how the Hawaiian bobtail squid finds and keeps its bioluminescent bacterial partner.Nature reviews. Microbiology · 2021Review
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
- Experimentally-validated correlation analysis reveals new anaerobic methane oxidation partnerships with consortium-level heterogeneity in diazotrophy.The ISME journal · 2021Article
- Article
- The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.PLoS biology · 2020Article
- Article
- Upregulation of virulence genes promotesProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- ThemSystems · 2019Article
- Involvement of a host Cathepsin L in symbiont-induced cell death.MicrobiologyOpen · 2018Article
- Characterization of the cell polarity gene crumbs during the early development and maintenance of the squid-vibrio light organ symbiosis.Development genes and evolution · 2017Article
- Stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells.PLoS genetics · 2017Article
- Mapping a multiplexed zoo of mRNA expression.Development (Cambridge, England) · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The establishment of a productive symbiosis between Euprymna scolopes, the Hawaiian bobtail squid, and its luminous bacterial symbiont, Vibrio fischeri, is mediated by transcriptional changes in both partners. A key challenge to unraveling the steps required to successfully initiate this and many other symbiotic associations is characterization of the timing and location of these changes. We report on the adaptation of hybridization chain reaction-fluorescent in situ hybridization (HCR-FISH) to simultaneously probe the spatiotemporal regulation of targeted genes in both E. scolopes and V. fischeri. This method revealed localized, transcriptionally coregulated epithelial cells within the light organ that responded directly to the presence of bacterial cells while, at the same time, provided a sensitive means to directly show regulated gene expression within the symbiont population. Thus, HCR-FISH provides a new approach for characterizing habitat transition in bacteria and for discovering host tissue responses to colonization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.