ArticleExperimental biology and medicine (Maywood, N.J.)2016
Advanced glycation end-products and insulin signaling in granulosa cells.
Article in Experimental biology and medicine (Maywood, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 66 citations in OpenAlex.
- Integrative Transcriptomic Analysis and Functional Validation Implicate GPT2 in Glycolytic Regulation in Polycystic Ovary Syndrome.Food science & nutrition · 2026Article
- Article
- SIRT3 attenuates AGEs-induced senescence in human granulosa cells through enhancing mitophagy.Cell biology and toxicology · 2026Article
- Are Dietary and Serum Advanced Glycation End Products (AGEs) Potential Contributors to Inflammation in Women with Polycystic Ovary Syndrome?Journal of clinical medicine · 2025Article
- The pathogenesis, therapeutic targets and drugs of polycystic ovary syndrome.Frontiers in endocrinology · 2025Review
- Metabolic state and female fertility in ART cycles: a summary of current advances.Frontiers in cell and developmental biology · 2025Review
- Advanced glycation end products and reactive oxygen species: uncovering the potential role of ferroptosis in diabetic complications.Molecular medicine (Cambridge, Mass.) · 2024Review
- Alpha-Lipoic Acid Ameliorates Impaired Steroidogenesis in Human Granulosa Cells Induced by Advanced Glycation End-Products.Iranian journal of medical sciences · 2024Article
- The role of vitamin D3 in follicle development.Journal of ovarian research · 2024Review
- Assessment of the dual role ofFrontiers in pharmacology · 2023Article
- Review
- Metabolic and Molecular Mechanisms of Diet and Physical Exercise in the Management of Polycystic Ovarian Syndrome.Biomedicines · 2022Review
- Gene cascade analysis in human granulosa tumor cells (KGN) following exposure to high levels of free fatty acids and insulin.Journal of ovarian research · 2021Article
- TMT-based proteomic and bioinformatic analyses of human granulosa cells from obese and normal-weight female subjects.Reproductive biology and endocrinology : RB&E · 2021Article
- In Search of New Therapeutics-Molecular Aspects of the PCOS Pathophysiology: Genetics, Hormones, Metabolism and Beyond.International journal of molecular sciences · 2020Review
- Dietary Glycotoxins, Advanced Glycation End Products, Inhibit Cell Proliferation and Progesterone Secretion in Ovarian Granulosa Cells and Mimic PCOS-like Symptoms.Biomolecules · 2019Article
- Advanced glycation end products alter steroidogenic gene expression by granulosa cells: an effect partially reversible by vitamin D.Molecular human reproduction · 2018Article
- Role of hormonal and inflammatory alterations in obesity-related reproductive dysfunction at the level of the hypothalamic-pituitary-ovarian axis.Reproductive biology and endocrinology : RB&E · 2018Review
- Overexpression of Lnk in the Ovaries Is Involved in Insulin Resistance in Women With Polycystic Ovary Syndrome.Endocrinology · 2016Article
- Insulin signalling and glucose transport in the ovary and ovarian function during the ovarian cycle.The Biochemical journal · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advanced glycation end-products (AGEs) may interfere with insulin intracellular signaling and glucose transport in human granulosa cells, potentially affecting ovarian function, follicular growth, linked with diminished fertility. The potential interaction of AGEs with insulin signaling pathways and glucose transport was investigated in human granulosa KGN cells. KGN cells were cultured with variable concentrations of human glycated albumin (HGA, 50-200 µg/mL) or insulin (100 ng/mL). Combined treatments of KGN cells with insulin (100 ng/mL) and HGA (200 µg/mL) were also performed. p-AKT levels and glucose transporter type 4 (Glut-4) translocation analysis were performed by Western blot. Phosphatidylinositol-3-kinase (PI3K)-specific signaling was checked by using the PI3K-inhibitor, LY294002. p-AKT levels were significantly increased following insulin treatment compared to basal levels or HGA exposure. This insulin-mediated AKT-phosphorylation was PI3K-specific and it was inhibited after combined treatment of insulin and HGA. Furthermore, Glut-4 translocation from the cytoplasm to the membrane compartments of KGN cells was remarkably reduced after the combined treatment of insulin and HGA. The present findings support that AGEs interfere with insulin signaling in granulosa cells and prevent Glut-4 membrane translocation suggesting that intra ovarian AGEs accumulation, from endogenous or exogenous sources, may contribute to the pathophysiology of states characterized with anovulation and insulin resistance such as polycystic ovary syndrome.
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