ArticleNucleic acids research2015
YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 131 citations in OpenAlex.
- TargetMITO: a rule-based model for generating functional synthetic mitochondrial targeting sequences in yeast.Nucleic acids research · 2026Article
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Integrating Machine Learning and Transcriptomics to Enhance β‑Carotene Production inACS omega · 2026Article
- A hybrid system enables plasmid copy number control in yeast.Nature communications · 2026Article
- Protocol for connector-enabled multigene pathway assembly using Golden Gate Assembly and yeast recombination.STAR protocols · 2026Article
- Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways.Microbial biotechnology · 2026Article
- The evolutionary path of widespread IR heteroplasmy of chloroplast genomes in the context of Rhodophyta phylogeny.Journal of molecular evolution · 2026Article
- Enhanced lycopene fortification in eggs using poultry feed containing a carotenoid-oil preparation derived from metabolically engineered baker's yeast.Scientific reports · 2026Article
- A modular synthetic biology toolkit unlocks metabolic engineering of the industrially relevant alga Nannochloropsis.Advanced biotechnology · 2026Article
- Biological parts in yeast synthetic biology: from regulatory elements to predictive design platforms.FEMS yeast research · 2026Review
- Probiotic-Based Materials as Living Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- AsAP2 transcriptionally activates ferulate 5-hydroxylase, diverting ferulic acid metabolism toward lignin biosynthesis in Angelica sinensis.Scientific reports · 2025Article
- Engineering COInternational journal of molecular sciences · 2025Article
- Synthetic collinearization of a chromosome segment enables genetic dissection of incompatibility between distant genera.bioRxiv : the preprint server for biology · 2025Article
- High-throughput profiling of chemical-induced gene expression across 93,644 perturbations.Nature methods · 2025Article
- Iterative SCRaMbLE for engineering synthetic genome modules and chromosomes.Nature communications · 2025Article
- Systematic Engineering for Efficient Uric Acid-Degrading Activity in Probiotic YeastACS synthetic biology · 2025Article
- Article
- Recent technology advancements in large-scale DNA assembly.Quantitative biology (Beijing, China) · 2025Article
- Advancements in Golden Gate Cloning: A Comprehensive Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
Abstract
It is a routine task in metabolic engineering to introduce multicomponent pathways into a heterologous host for production of metabolites. However, this process sometimes may take weeks to months due to the lack of standardized genetic tools. Here, we present a method for the design and construction of biological parts based on the native genes and regulatory elements in Saccharomyces cerevisiae. We have developed highly efficient protocols (termed YeastFab Assembly) to synthesize these genetic elements as standardized biological parts, which can be used to assemble transcriptional units in a single-tube reaction. In addition, standardized characterization assays are developed using reporter constructs to calibrate the function of promoters. Furthermore, the assembled transcription units can be either assayed individually or applied to construct multi-gene metabolic pathways, which targets a genomic locus or a receiving plasmid effectively, through a simple in vitro reaction. Finally, using β-carotene biosynthesis pathway as an example, we demonstrate that our method allows us not only to construct and test a metabolic pathway in several days, but also to optimize the production through combinatorial assembly of a pathway using hundreds of regulatory biological parts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.