ArticleParasitology research2015
Identification and characterization of a microneme protein (NcMIC6) in Neospora caninum.
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Unveiling the impact of cGMP-dependent protein kinase of Neospora caninum on calcium fluxes and egress functions through quantitative phosphoproteome analysis.Communications biology · 2025Article
- Immunization with the NcMYR1 gene knockout strain effectively protected C57BL/6 mice and their pups against theVirulence · 2024Article
- Identification and Characterization ofVeterinary sciences · 2022Article
- Characterization of the Eimeria tenella rhoptry protein with a nuclear localization sequence (EtROP30).Parasitology research · 2022Article
- Towards the First Multiepitope Vaccine Candidate againstBioMed research international · 2022Article
- Determination of B and T Cell Epitopes inBioMed research international · 2022Article
- Neospora caninum SRS2 Protein: Essential Vaccination Targets and Biochemical Features for Next-Generation Vaccine Design.BioMed research international · 2022Article
- Evaluation of 4 merozoite antigens as candidate vaccines against Eimeria tenella infection.Poultry science · 2021Article
- Microneme Protein 6 Is Involved in Invasion and Egress byPathogens (Basel, Switzerland) · 2021Article
- Diverse Roles of TgMIC1/4/6 in theFrontiers in microbiology · 2021Review
- Article
- From Signaling Pathways to Distinct Immune Responses: Key Factors for Establishing or CombatingPathogens (Basel, Switzerland) · 2020Review
- Functional characterization of acyl-CoA binding protein in Neospora caninum.Parasites & vectors · 2020Article
- Glutaredoxin 1 Deficiency Leads to Microneme Protein-Mediated Growth Defects inFrontiers in microbiology · 2020Article
- Characterization ofFrontiers in veterinary science · 2020Article
- New molecular tools in Neospora caninum for studying apicomplexan parasite proteins.Scientific reports · 2017Article
- The p38 MAPK inhibitor, SB203580, inhibits cell invasion by Neospora caninum.Parasitology research · 2017Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neospora caninum, an Apicomplexa parasite, is the causative agent of neosporosis. As described for other members of Apicomplexa, microneme proteins (MICs) play a key role in attachment and invasion of host cells by N. caninum. Herein we identified N. caninum microneme protein 6 (NcMIC6) that is orthologous to Toxoplasma gondii microneme protein 6 (TgMIC6). The open reading frame of the NcMIC6 gene is 984 bp and encodes a 327 amino acid peptide. Sequence analysis showed that NcMIC6 included a signal peptide, a transmembrane region, three epidermal growth factor-like (EGF) domains, and two low complexity regions. Antibodies raised against recombinant NcMIC6 recognized an approximately 35-kDa native MIC6 protein in Western blots of N. caninum tachyzoites. Immunofluorescence analysis showed that NcMIC6 had a polar labeling pattern, which was consistent with localization of micronemes in the apical region. Pulse invasion assays showed that NcMIC6 translocated from the apical tip to the posterior end of the parasites. Secretion assays demonstrated that NcMIC6 was released into the supernatants. Importantly, it was clearly revealed by co-immunoprecipitation that NcMIC6 formed a complex with other two soluble microneme proteins (NcMIC1 and NcMIC4). In conclusion, identification and characterization of the novel microneme protein NcMIC6 may contribute to understanding how this protein functions during the parasite motility and host cell invasion.
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