ArticleInternational journal of molecular sciences2015
Gene Overexpression and RNA Silencing Tools for the Genetic Manipulation of the S-(+)-Abscisic Acid Producing Ascomycete Botrytis cinerea.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Metabolic reprogramming of abscisic acid-producing strainSynthetic and systems biotechnology · 2026Article
- Heterologous expression of the hypovirus CHV1-EP713 full-length cDNA in Botrytis cinerea: transformation with Agrobacterium tumefaciens and evaluation of changes in the fungal phenotype.Biological research · 2025Article
- RNA Interference in Fungal Plant Pathogens: What Do We Know fromJournal of fungi (Basel, Switzerland) · 2025Review
- Enhancing abscisic acid production inSynthetic and systems biotechnology · 2025Article
- Mechanisms and Impact of Rhizosphere Microbial Metabolites on Crop Health, Traits, Functional Components: A Comprehensive Review.Molecules (Basel, Switzerland) · 2024Review
- Engineering strategies for enhanced 1', 4'-trans-ABA diol production by Botrytis cinerea.Microbial cell factories · 2024Article
- Normal distribution of H3K9me3 occupancy co-mediated by histone methyltransferase BcDIM5 and histone deacetylase BcHda1 maintains stable ABA synthesis inFrontiers in microbiology · 2024Article
- Production of abscisic acid in the oleaginous yeast Yarrowia lipolytica.FEMS yeast research · 2022Article
- Deciphering immune responses primed by a bacterial lipopeptide in wheat towardsFrontiers in plant science · 2022Article
- Combined transcriptome and proteome analysis of Bcfrp1 involved in regulating the biosynthesis of abscisic acid and growth inFrontiers in microbiology · 2022Article
- The BcLAE1 is involved in the regulation of ABA biosynthesis inFrontiers in microbiology · 2022Article
- CysApplied and environmental microbiology · 2018Article
- Abscisic Acid as Pathogen Effector and Immune Regulator.Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phytopathogenic ascomycete Botrytis cinerea produces several secondary metabolites that have biotechnical significance and has been particularly used for S-(+)-abscisic acid production at the industrial scale. To manipulate the expression levels of specific secondary metabolite biosynthetic genes of B. cinerea with Agrobacterium tumefaciens-mediated transformation system, two expression vectors (pCBh1 and pCBg1 with different selection markers) and one RNA silencing vector, pCBSilent1, were developed with the In-Fusion assembly method. Both expression vectors were highly effective in constitutively expressing eGFP, and pCBSilent1 effectively silenced the eGFP gene in B. cinerea. Bcaba4, a gene suggested to participate in ABA biosynthesis in B. cinerea, was then targeted for gene overexpression and RNA silencing with these reverse genetic tools. The overexpression of bcaba4 dramatically induced ABA formation in the B. cinerea wild type strain Bc-6, and the gene silencing of bcaba4 significantly reduced ABA-production in an ABA-producing B. cinerea strain.
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Registered trials
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