ArticlePloS one2015
Transportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Enhanced secretion of extracellular proteins inMycology · 2026Article
- Revisiting fungal polarized growth: from Spitzenkörper to crescent accumulation of secretory vesicles.iScience · 2025Article
- A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling.Communications biology · 2024Article
- The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans.Current genetics · 2023Article
- Chitin Biosynthesis inJournal of fungi (Basel, Switzerland) · 2023Review
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Article
- The Myosin Motor Domain-Containing Chitin Synthases Are Involved in Cell Wall Integrity and Sensitivity to Antifungal Proteins inFrontiers in microbiology · 2019Article
- Secretory Vesicle Polar Sorting, Endosome Recycling and Cytoskeleton Organization Require the AP-1 Complex inGenetics · 2018Article
- Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures.Microbiology and molecular biology reviews : MMBR · 2018Review
- Endocytic recycling via the TGN underlies the polarized hyphal mode of life.PLoS genetics · 2018Article
- Superresolution and pulse-chase imaging reveal the role of vesicle transport in polar growth of fungal cells.Science advances · 2018Article
- Pulses of CaProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transport of fungal RAB11 secretory vesicles involves myosin-5, dynein/dynactin/p25, and kinesin-1 and is independent of kinesin-3.Molecular biology of the cell · 2017Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Co-delivery of cell-wall-forming enzymes in the same vesicle for coordinated fungal cell wall formation.Nature microbiology · 2016Article
- Dynamics of Actin Cables in Polarized Growth of the Filamentous Fungus Aspergillus nidulans.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell wall formation and maintenance are crucial for hyphal morphogenesis. In many filamentous fungi, chitin is one of the main structural components of the cell wall. Aspergillus nidulans ChsB, a chitin synthase, and CsmA, a chitin synthase with a myosin motor-like domain (MMD) at its N-terminus, both localize predominantly at the hyphal tip regions and at forming septa. ChsB and CsmA play crucial roles in polarized hyphal growth in A. nidulans. In this study, we investigated the mechanism by which CsmA and ChsB accumulate at the hyphal tip in living hyphae. Deletion of kinA, a gene encoding conventional kinesin (kinesin-1), impaired the localization of GFP-CsmA and GFP-ChsB at the hyphal tips. The transport frequency of GFP-CsmA and GFP-ChsB in both anterograde and retrograde direction appeared lower in the kinA-deletion strain compared to wild type, although the velocities of the movements were comparable. Co-localization of GFP-ChsB and GFP-CsmA with mRFP1-KinArigor, a KinA mutant that binds to microtubules but does not move along them, was observed in the posterior of the hyphal tip regions. KinA co-immunoprecipitated with ChsB and CsmA. Co-localization and association of CsmA with KinA did not depend on the MMD. These findings indicate that ChsB and CsmA are transported along microtubules to the subapical region by KinA.
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