ArticleBiophysical journal2015
MPP1 as a Factor Regulating Phase Separation in Giant Plasma Membrane-Derived Vesicles.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Review
- EFR3A: a new raft domain organizing protein?Cellular & molecular biology letters · 2023Article
- High-Content Imaging Platform to Discover Chemical Modulators of Plasma Membrane Rafts.ACS central science · 2022Article
- MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules.Cells · 2022Article
- Article
- Molecular characterization of direct interactions between MPP1 and flotillins.Scientific reports · 2021Article
- Not Just Another Scaffolding Protein Family: The Multifaceted MPPs.Molecules (Basel, Switzerland) · 2020Review
- Peripheral myelin protein 22 preferentially partitions into ordered phase membrane domains.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Flotillins: At the Intersection of ProteinInternational journal of molecular sciences · 2020Review
- The microdomain-organizing protein MPP1 is required for insulin-stimulated activation of H-Ras.Oncotarget · 2018Article
- Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA.The British journal of nutrition · 2018Article
- Miscibility Transition Temperature Scales with Growth Temperature in a Zebrafish Cell Line.Biophysical journal · 2017Article
- Emerging role of chemoprotective agents in the dynamic shaping of plasma membrane organization.Biochimica et biophysica acta. Biomembranes · 2017Review
- The mystery of membrane organization: composition, regulation and roles of lipid rafts.Nature reviews. Molecular cell biology · 2017Review
- The Continuing Mystery of Lipid Rafts.Journal of molecular biology · 2016Review
- Polyunsaturated Lipids Regulate Membrane Domain Stability by Tuning Membrane Order.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The existence of membrane-rafts helps to conceptually understand the spatiotemporal organization of membrane-associated events (signaling, fusion, fission, etc.). However, as rafts themselves are nanoscopic, dynamic, and transient assemblies, they cannot be directly observed in a metabolizing cell by traditional microscopy. The observation of phase separation in giant plasma membrane-derived vesicles from live cells is a powerful tool for studying lateral heterogeneity in eukaryotic cell membranes, specifically in the context of membrane rafts. Microscopic phase separation is detectable by fluorescent labeling, followed by cooling of the membranes below their miscibility phase transition temperature. It remains unclear, however, if this lipid-driven process is tuneable in any way by interactions with proteins. Here, we demonstrate that MPP1, a member of the MAGUK family, can modulate membrane properties such as the fluidity and phase separation capability of giant plasma membrane-derived vesicles. Our data suggest that physicochemical domain properties of the membrane can be modulated, without major changes in lipid composition, through proteins such as MPP1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.