ArticleBiophysical journal2015
Human Primary Immune Cells Exhibit Distinct Mechanical Properties that Are Modified by Inflammation.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed.
- Actin conformation dynamics precede force generation in cytotoxic T lymphocytes.Communications biology · 2026Article
- Decoding mechanoregulation in immunological synapses using biomimetic artificial cells.Nature methods · 2026Article
- Simulation-Based Microfluidic Deformation Mapping for Region-Dependent Apparent Young's Modulus Estimation of Single Cells.Biosensors · 2026Article
- Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription.Nature communications · 2026Article
- Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Safe and Efficient CRISPR Genome Editing of Primary Human T Cells Using a Droplet-Based Cell Mechanoporation Platform.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Cytokine-Engineered Chimeric Antigen Receptor-T Cell Therapy: How to Balance the Efficacy and Toxicity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Computational prediction of cellular elastic modulus from mechanosensitive gene expression at multiple biological levels.BioData mining · 2025Article
- B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses.FEBS letters · 2025Review
- Immune Cell Migration Models Synergize Nuclear Piston, Uropod, and Microenvironment into Hydraulic Cell Engine.bioRxiv : the preprint server for biology · 2025Article
- Immunological synapse: structures, molecular mechanisms and therapeutic implications in disease.Signal transduction and targeted therapy · 2025Review
- Platforms for studying cell-cell recognition by immune cells.Immunology and cell biology · 2025Review
- Soft matter mechanics of immune cell aggregates.Journal of the Royal Society, Interface · 2025Article
- Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes.Nature communications · 2025Article
- Antigen mobility regulates the dynamics and precision of antigen capture in the B cell immune synapse.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bridging hemodynamics, tissue mechanics, and pathophysiology in coronary artery disease: A new agent-based model with tetrahedral mesh integration.Journal of biomechanics · 2025Article
- Single-cell electro-mechanical shear flow deformability cytometry.Microsystems & nanoengineering · 2024Article
- β2 integrins impose a mechanical checkpoint on macrophage phagocytosis.Nature communications · 2024Article
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Elasticity of Carrier Fluid: A Key Factor Affecting Mechanical Phenotyping in Deformability Cytometry.Micromachines · 2024Article
33 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T lymphocytes are key modulators of the immune response. Their activation requires cell-cell interaction with different myeloid cell populations of the immune system called antigen-presenting cells (APCs). Although T lymphocytes have recently been shown to respond to mechanical cues, in particular to the stiffness of their environment, little is known about the rigidity of APCs. In this study, single-cell microplate assays were performed to measure the viscoelastic moduli of different human myeloid primary APCs, i.e., monocytes (Ms, storage modulus of 520 +90/-80 Pa), dendritic cells (DCs, 440 +110/-90 Pa), and macrophages (MPHs, 900 +110/-100 Pa). Inflammatory conditions modulated these properties, with storage moduli ranging from 190 Pa to 1450 Pa. The effect of inflammation on the mechanical properties was independent of the induction of expression of commonly used APC maturation markers, making myeloid APC rigidity an additional feature of inflammation. In addition, the rigidity of human T lymphocytes was lower than that of all myeloid cells tested and among the lowest reported (Young's modulus of 85 ± 5 Pa). Finally, the viscoelastic properties of myeloid cells were dependent on both their filamentous actin content and myosin IIA activity, although the relative contribution of these parameters varied within cell types. These results indicate that T lymphocytes face different cell rigidities when interacting with myeloid APCs in vivo and that this mechanical landscape changes under inflammation.
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