ArticleEvoDevo2015
The Nodal signaling pathway controls left-right asymmetric development in amphioxus.
Article in EvoDevo, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- From Petri Dish to Primitive Heart: How IVF Alters Early Cardiac Gene Networks and Epigenetic Landscapes.Biomedicines · 2025Review
- Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head.Nature communications · 2024Article
- Evolution of the gene regulatory network of body axis by enhancer hijacking in amphioxus.eLife · 2024Article
- A Small Change With a Twist Ending: A Single Residue in EGF-CFC Drives Bilaterian Asymmetry.Molecular biology and evolution · 2023Article
- Differentiation of endostyle cells by Nkx2-1 and FoxE in the ascidian Ciona intestinalis type A: insights into shared gene regulation in glandular- and thyroid-equivalent elements of the chordate endostyle.Cell and tissue research · 2022Article
- Article
- Hedgehog signaling controls mouth opening in the amphioxus.Zoological letters · 2021Article
- Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development.eLife · 2021Article
- Pitx controls amphioxus asymmetric morphogenesis by promoting left-side development and repressing right-side formation.BMC biology · 2021Article
- Disruption of left-right axis specification in Ciona induces molecular, cellular, and functional defects in asymmetric brain structures.BMC biology · 2021Article
- Lineage analysis reveals an endodermal contribution to the vertebrate pituitary.Science (New York, N.Y.) · 2020Article
- Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer.eLife · 2020Article
- Article
- Multiple Roles of Pitx2 in Cardiac Development and Disease.Journal of cardiovascular development and disease · 2017Review
- Nitric Oxide regulates mouth development in amphioxus.Scientific reports · 2017Article
- Evolutionary history of the extant amphioxus lineage with shallow-branching diversification.Scientific reports · 2017Article
- Cerberus-Nodal-Lefty-Pitx signaling cascade controls leftProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Nodal and BMP expression during the transition to pentamery in the sea urchin Heliocidaris erythrogramma: insights into patterning the enigmatic echinoderm body plan.BMC developmental biology · 2017Article
- Article
- Nodal signalling and asymmetry of the nervous system.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNodal is an important determinant of the left-right (LR) body axis in bilaterians, specifying the right side in protostomes and non-chordate deuterostomes as opposed to the left side in chordates. Amphioxus represents an early-branching chordate group, rendering it especially useful for studying the character states that predate the origin of vertebrates. However, its anatomy, involving offset arrangement of axial structures, marked asymmetry of the oropharyngeal region, and, most notably, a mouth positioned on the left side, contrasts with the symmetric arrangement of the corresponding regions in other chordates.
resultsWe show that the Nodal signaling pathway acts to specify the LR axis in the cephalochordate amphioxus in a similar way as in vertebrates. At early neurula stages, Nodal switches from initial bilateral to the left-sided expression and subsequently specifies the left embryonic side. Perturbation of Nodal signaling with small chemical inhibitors (SB505124 and SB431542) alters expression of other members of the pathway and of left/right-sided, organ-specific genes. Upon inhibition, larvae display loss of the innate alternation of both somites and axons of peripheral nerves and loss of left-sided pharyngeal structures, such as the mouth, the preoral pit, and the duct of the club-shaped gland. Concomitantly, the left side displays ectopic expression of otherwise right-sided genes, and the larvae exhibit bilaterally symmetrical morphology, with duplicated endostyle and club-shaped gland structures.
conclusionsWe demonstrate that Nodal signaling is necessary for establishing the LR embryonic axis and for developing profound asymmetry in amphioxus. Our data suggest that initial symmetry breaking in amphioxus and propagation of the pathway on the left side correspond with the situation in vertebrates. However, the organs that become targets of the pathway differ between amphioxus and vertebrates, which may explain the pronounced asymmetry of its oropharyngeal and axial structures and the left-sided position of the mouth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.