ArticleFrontiers in plant science2015
Knockin' on pollen's door: live cell imaging of early polarization events in germinating Arabidopsis pollen.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Unconventional vesicle transport driven by formin-actin-myosin working system during pollen germination.Science advances · 2026Article
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Three-dimensional visualization of tip-vesicles in growing pollen tubes by electron tomography.Science China. Life sciences · 2026Article
- Bioimaging insights into structural pathways of cell-to-cell communication within the male (MGU) and female (FGU) germ units of Arabidopsis thaliana.Plant cell reports · 2025Article
- A land plant-specific VPS13 mediates polarized vesicle trafficking in germinating pollen.The New phytologist · 2025Article
- The male germ unit association is independently regulated of GUM inPlant direct · 2024Article
- Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen.Nature communications · 2024Article
- Transcriptomic Insight into the Pollen Tube Growth ofPlants (Basel, Switzerland) · 2023Article
- Thermosensitivity of pollen: a molecular perspective.Plant cell reports · 2023Review
- A Decade of Pollen Phosphoproteomics.International journal of molecular sciences · 2021Review
- Live-cell imaging of early events following pollen perception in self-incompatible Arabidopsis thaliana.Journal of experimental botany · 2020Article
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- Male gametophyte development and function in angiosperms: a general concept.Plant reproduction · 2016Review
- Phosphoproteomics Profiling of Tobacco Mature Pollen and Pollen Activated in vitro.Molecular & cellular proteomics : MCP · 2016Article
- Actin3 promoter reveals undulating F-actin bundles at shanks and dynamic F-actin meshworks at tips of tip-growing pollen tubes.Plant signaling & behavior · 2016Article
- F-actin forms mobile and unwinding ring-shaped structures in germinating Arabidopsis pollen expressing Lifeact.Plant signaling & behavior · 2015Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen tubes are an excellent system for studying the cellular dynamics and complex signaling pathways that coordinate polarized tip growth. Although several signaling mechanisms acting in the tip-growing pollen tube have been described, our knowledge on the subcellular and molecular events during pollen germination and growth site selection at the pollen plasma membrane is rather scarce. To simultaneously track germinating pollen from up to 12 genetically different plants we developed an inexpensive and easy mounting technique, suitable for every standard microscope setup. We performed high magnification live-cell imaging during Arabidopsis pollen activation, germination, and the establishment of pollen tube tip growth by using fluorescent marker lines labeling either the pollen cytoplasm, vesicles, the actin cytoskeleton or the sperm cell nuclei and membranes. Our studies revealed distinctive vesicle and F-actin polarization during pollen activation and characteristic growth kinetics during pollen germination and pollen tube formation. Initially, the germinating Arabidopsis pollen tube grows slowly and forms a uniform roundish bulge, followed by a transition phase with vesicles heavily accumulating at the growth site before switching to rapid tip growth. Furthermore, we found the two sperm cells to be transported into the pollen tube after the phase of rapid tip growth has been initiated. The method presented here is suitable to quantitatively study subcellular events during Arabidopsis pollen germination and growth, and for the detailed analysis of pollen mutants with respect to pollen polarization, bulging, or growth site selection at the pollen plasma membrane.
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