ArticleFrontiers in plant science2015
Loss of the Arabidopsis thaliana P4-ATPases ALA6 and ALA7 impairs pollen fitness and alters the pollen tube plasma membrane.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Functional Characterization ofBiology · 2026Article
- Article
- Understanding the role of P-type ATPases in regulating pollen fertility and development in pigeonpea.Molecular genetics and genomics : MGG · 2024Article
- Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress.Journal of experimental botany · 2023Article
- Mini-review: Lipid flippases as putative targets for biotechnological crop improvement.Frontiers in plant science · 2023Review
- The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis.The Plant cell · 2022Article
- Imaging of Lipid Uptake inBio-protocol · 2021Article
- Arabidopsis P4 ATPase-mediated cell detoxification confers resistance to Fusarium graminearum and Verticillium dahliae.Nature communications · 2021Article
- Lipid flippases as key players in plant adaptation to their environment.Nature plants · 2021Review
- Dynamic membranes: the multiple roles of P4 and P5 ATPases.Plant physiology · 2021Article
- A potential pathway for flippase-facilitated glucosylceramide catabolism in plants.Plant signaling & behavior · 2020Article
- Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.The Plant cell · 2020Article
- The Lipid Flippases ALA4 and ALA5 Play Critical Roles in Cell Expansion and Plant Growth.Plant physiology · 2020Article
- Article
- An Overview of Biomembrane Functions in Plant Responses to High-Temperature Stress.Frontiers in plant science · 2018Review
- ALA6, a PFrontiers in plant science · 2017Article
- Acetolactate Synthase-Inhibiting Gametocide Amidosulfuron Causes Chloroplast Destruction, Tissue Autophagy, and Elevation of Ethylene Release in Rapeseed.Frontiers in plant science · 2017Article
- Article
- ALA10, a Phospholipid Flippase, Controls FAD2/FAD3 Desaturation of Phosphatidylcholine in the ER and Affects Chloroplast Lipid Composition in Arabidopsis thaliana.Plant physiology · 2016Article
- A phospholipid uptake system in the model plant Arabidopsis thaliana.Nature communications · 2015Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Members of the P4 subfamily of P-type ATPases are thought to create and maintain lipid asymmetry in biological membranes by flipping specific lipids between membrane leaflets. In Arabidopsis, 7 of the 12 Aminophospholipid ATPase (ALA) family members are expressed in pollen. Here we show that double knockout of ALA6 and ALA7 (ala6/7) results in siliques with a ~2-fold reduction in seed set with a high frequency of empty seed positions near the bottom. Seed set was reduced to near zero when plants were grown under a hot/cold temperature stress. Reciprocal crosses indicate that the ala6/7 reproductive deficiencies are due to a defect related to pollen transmission. In-vitro growth assays provide evidence that ala6/7 pollen tubes are short and slow, with ~2-fold reductions in both maximal growth rate and overall length relative to wild-type. Outcrosses show that when ala6/7 pollen are in competition with wild-type pollen, they have a near 0% success rate in fertilizing ovules near the bottom of the pistil, consistent with ala6/7 pollen having short and slow growth defects. The ala6/7 phenotypes were rescued by the expression of either an ALA6-YFP or GFP-ALA6 fusion protein, which showed localization to both the plasma membrane and highly-mobile endomembrane structures. A mass spectrometry analysis of mature pollen grains revealed significant differences between ala6/7 and wild-type, both in the relative abundance of lipid classes and in the average number of double bonds present in acyl side chains. A change in the properties of the ala6/7 plasma membrane was also indicated by a ~10-fold reduction of labeling by lipophilic FM-dyes relative to wild-type. Together, these results indicate that ALA6 and ALA7 provide redundant activities that function to directly or indirectly change the distribution and abundance of lipids in pollen, and support a model in which ALA6 and ALA7 are critical for pollen fitness under normal and temperature-stress conditions.
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