ArticleFrontiers in microbiology2015
Forest floor community metatranscriptomes identify fungal and bacterial responses to N deposition in two maple forests.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 109 citations in OpenAlex.
- Pooled it
- Microaggregates as Nutrient Reservoirs for Fungi Drive Natural Regeneration in Larch Plantation Forests.Journal of fungi (Basel, Switzerland) · 2025Article
- Applying molecular and genetic methods to trees and their fungal communities.Applied microbiology and biotechnology · 2023Review
- Fungal communities in soils under global change.Studies in mycology · 2022Article
- Arbuscular Mycorrhizal Tree Communities Have Greater Soil Fungal Diversity and Relative Abundances of Saprotrophs and Pathogens than Ectomycorrhizal Tree Communities.Applied and environmental microbiology · 2022Article
- Microbial community composition controls carbon flux across litter types in early phase of litter decomposition.Environmental microbiology · 2021Article
- Comparative Fungal Community Analyses Using Metatranscriptomics and Internal Transcribed Spacer Amplicon Sequencing from Norway Spruce.mSystems · 2021Article
- Effects of Nitrogen Deposition on Nitrogen-Mineralizing Enzyme Activity and Soil Microbial Community Structure in a Korean Pine Plantation.Microbial ecology · 2021Article
- Complementary Roles of Wood-Inhabiting Fungi and Bacteria Facilitate Deadwood Decomposition.mSystems · 2021Article
- Metatranscriptomics: an approach for retrieving novel eukaryotic genes from polluted and related environments.3 Biotech · 2020Review
- Targeted Metagenomics of Retting in Flax: The Beginning of the Quest to Harness the Secret Powers of the Microbiota.Frontiers in genetics · 2020Review
- Mycobiome diversity: high-throughput sequencing and identification of fungi.Nature reviews. Microbiology · 2019Review
- Soil Metatranscriptomes Under Long-Term Experimental Warming and Drying: Fungi Allocate Resources to Cell Metabolic Maintenance Rather Than Decay.Frontiers in microbiology · 2019Article
- Nitrogen additions stimulate litter humification in a subtropical forest, southwestern China.Scientific reports · 2018Article
- Multi-omic Analyses of Extensively Decayed Pinus contorta Reveal Expression of a Diverse Array of Lignocellulose-Degrading Enzymes.Applied and environmental microbiology · 2018Article
- Short-Term Transcriptional Response of Microbial Communities to Nitrogen Fertilization in a Pine Forest Soil.Applied and environmental microbiology · 2018Article
- Anthropogenic N Deposition Alters the Composition of Expressed Class II Fungal Peroxidases.Applied and environmental microbiology · 2018Article
- Belowground Microbiota and the Health of Tree Crops.Frontiers in microbiology · 2018Review
- Multiomic Strategies Reveal Diversity and Important Functional Aspects of Human Gut Microbiome.BioMed research international · 2018Article
- Plant, fungal, bacterial, and nitrogen interactions in the litter layer of a native Patagonian forest.PeerJ · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anthropogenic N deposition alters patterns of C and N cycling in temperate forests, where forest floor litter decomposition is a key process mediated by a diverse community of bacteria and fungi. To track forest floor decomposer activity we generated metatranscriptomes that simultaneously surveyed the actively expressed bacterial and eukaryote genes in the forest floor, to compare the impact of N deposition on the decomposers in two natural maple forests in Michigan, USA, where replicate field plots had been amended with N for 16 years. Site and N amendment responses were compared using about 74,000 carbohydrate active enzyme transcript sequences (CAZymes) in each metatranscriptome. Parallel ribosomal RNA (rRNA) surveys of bacterial and fungal biomass and taxonomic composition showed no significant differences in either biomass or OTU richness between the two sites or in response to N. Site and N amendment were not significant variables defining bacterial taxonomic composition, but they were significant for fungal community composition, explaining 17 and 14% of the variability, respectively. The relative abundance of expressed bacterial and fungal CAZymes changed significantly with N amendment in one of the forests, and N-response trends were also identified in the second forest. Although the two ambient forests were similar in community biomass, taxonomic structure and active CAZyme profile, the shifts in active CAZyme profiles in response to N-amendment differed between the sites. One site responded with an over-expression of bacterial CAZymes, and the other site responded with an over-expression of both fungal and different bacterial CAZymes. Both sites showed reduced representation of fungal lignocellulose degrading enzymes in N-amendment plots. The metatranscriptome approach provided a holistic assessment of eukaryote and bacterial gene expression and is applicable to other systems where eukaryotes and bacteria interact.
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Registered trials
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