Evidence map›Paper›PMID 25953104›Full record

ArticlePlant physiology2015

TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE-Mediated Generation and Metabolic Analysis of Camalexin-Deficient cyp71a12 cyp71a13 Double Knockout Lines.

Teresa M Müller, Christoph Böttcher, Robert Morbitzer, Cornelia C Götz, Johannes Lehmann, Thomas Lahaye, Erich Glawischnig

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
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  8. Review
  9. Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis inProceedings of the National Academy of Sciences of the United States of America · 2021
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  16. Root-specific camalexin biosynthesis controls the plant growth-promoting effects of multiple bacterial strains.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  17. Article
  18. Article
  19. GlutathioneFrontiers in plant science · 2018
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Teresa M MüllerLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.).
Christoph BöttcherLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.).
Robert MorbitzerLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.).
Cornelia C GötzLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.).
Johannes LehmannLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.).
Thomas LahayeLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.).
Erich GlawischnigLehrstuhl für Genetik, Technische Universität München, 85354 Freising, Germany (T.M.M., C.C.G., J.L., E.G.);Julius Kühn-Institut, Institut für Ökologische Chemie, Pflanzenanalytik, und Vorratsschutz, 14195 Berlin, Germany (C.B.); andCenter for Plant Molecular Biology-General Genetics, University of Tübingen, 72076 Tuebingen, Germany (R.M., T.L.) egl@wzw.tum.de.
Technical University of Munich · DEUniversity of Tübingen · DEJulius Kühn-Institut · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In Arabidopsis (Arabidopsis thaliana), a number of defense-related metabolites are synthesized via indole-3-acetonitrile (IAN), including camalexin and indole-3-carboxylic acid (ICOOH) derivatives. Cytochrome P450 71A13 (CYP71A13) is a key enzyme for camalexin biosynthesis and catalyzes the conversion of indole-3-acetaldoxime (IAOx) to IAN. The CYP71A13 gene is located in tandem with its close homolog CYP71A12, also encoding an IAOx dehydratase. However, for CYP71A12, indole-3-carbaldehyde and cyanide were identified as major reaction products. To clarify CYP71A12 function in vivo and to better understand IAN metabolism, we generated two cyp71a12 cyp71a13 double knockout mutant lines. CYP71A12-specific transcription activator-like effector nucleases were introduced into the cyp71a13 background, and very efficient somatic mutagenesis was achieved. We observed stable transmission of the cyp71a12 mutation to the following generations, which is a major challenge for targeted mutagenesis in Arabidopsis. In contrast to cyp71a13 plants, in which camalexin accumulation is partially reduced, double mutants synthesized only traces of camalexin, demonstrating that CYP71A12 contributes to camalexin biosynthesis in leaf tissue. A major role of CYP71A12 was identified for the inducible biosynthesis of ICOOH. Specifically, the ICOOH methyl ester was reduced to 12% of the wild-type level in AgNO3-challenged cyp71a12 leaves. In contrast, indole-3-carbaldehyde derivatives apparently are synthesized via alternative pathways, such as the degradation of indole glucosinolates. Based on these results, we present a model for this surprisingly complex metabolic network with multiple IAN sources and channeling of IAOx-derived IAN into camalexin biosynthesis. In conclusion, transcription activator-like effector nuclease-mediated mutation is a powerful tool for functional analysis of tandem genes in secondary metabolism.

Indexed as

Gene Knockout TechniquesArabidopsisArabidopsis ProteinsBase SequenceCytochrome P-450 Enzyme SystemDeoxyribonucleasesIndolesInheritance PatternsMetabolomicsModels, BiologicalMolecular Sequence DataMutagenesisMutationOximesPhytoalexinsSecondary MetabolismArabidopsis Proteinscamalexincytochrome P-450 71A12, Arabidopsiscytochrome P-450 71A13 protein, ArabidopsisCytochrome P-450 Enzyme SystemDeoxyribonucleasesindole-3-acetaldoximeindole-3-acetonitrileIndolesOximesPhytoalexinsThiazolesTrans-Activators

Identifiers

PMID25953104
PMCPMC4741344
OpenAlexW1592052354

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.