ArticleIntegrative biology : quantitative biosciences from nano to macro2015
High-throughput screening for modulators of cellular contractile force.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The trial behind it
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Who cites it
35 citing papers in PubMed.
- Inferring cellular contractile forces and work using deep morphology traction microscopy.Biophysical journal · 2024Article
- A life off the beaten track in biomechanics: Imperfect elasticity, cytoskeletal glassiness, and epithelial unjamming.Biophysics reviews · 2023Review
- Surface Engineering of FLT4-Targeted Nanocarriers Enhances Cell-Softening Glaucoma Therapy.ACS applied materials & interfaces · 2021Article
- Quantifying cellular forces: Practical considerations of traction force microscopy for dermal fibroblasts.Experimental dermatology · 2021Review
- Therapeutic Drugs and Devices for Tackling Ocular Hypertension and Glaucoma, and Need for Neuroprotection and Cytoprotective Therapies.Frontiers in pharmacology · 2021Review
- Review
- Strategies for targeting the cardiac sarcomere: avenues for novel drug discovery.Expert opinion on drug discovery · 2020Review
- Tissue traction microscopy to quantify muscle contraction within precision-cut lung slices.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Airway smooth muscle tone increases actin filamentogenesis and contractile capacity.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening.Frontiers in bioengineering and biotechnology · 2020Review
- Inhibiting Airway Smooth Muscle Contraction Using Pitavastatin: A Role for the Mevalonate Pathway in Regulating Cytoskeletal Proteins.Frontiers in pharmacology · 2020Article
- Cellogram: On-the-Fly Traction Force Microscopy.Nano letters · 2019Article
- Article
- Anti-fibrotic effects of tannic acid through regulation of a sustained TGF-beta receptor signaling.Respiratory research · 2019Article
- Extracellular matrix stiffness regulates human airway smooth muscle contraction by altering the cell-cell coupling.Scientific reports · 2019Article
- High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition.Journal of visualized experiments : JoVE · 2019Article
- Probe Sensitivity to Cortical versus Intracellular Cytoskeletal Network Stiffness.Biophysical journal · 2019Article
- Multiplexed, high-throughput measurements of cell contraction and endothelial barrier function.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Cyclic stretch enhances reorientation and differentiation of 3-D culture model of human airway smooth muscle.Biochemistry and biophysics reports · 2018Article
- Airway epithelial compression promotes airway smooth muscle proliferation and contraction.American journal of physiology. Lung cellular and molecular physiology · 2018Article
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
When cellular contractile forces are central to pathophysiology, these forces comprise a logical target of therapy. Nevertheless, existing high-throughput screens are limited to upstream signalling intermediates with poorly defined relationships to such a physiological endpoint. Using cellular force as the target, here we report a new screening technology and demonstrate its applications using human airway smooth muscle cells in the context of asthma and Schlemm's canal endothelial cells in the context of glaucoma. This approach identified several drug candidates for both asthma and glaucoma. We attained rates of 1000 compounds per screening day, thus establishing a force-based cellular platform for high-throughput drug discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.