Evidence map›Paper›PMID 25952767›Full record

ArticleIn vitro cellular & developmental biology. Animal2015

Establishment of a cell line from the ash and privet borer beetle Tylonotus bimaculatus Haldeman and assessment of its sensitivity to diacylhydrazine insecticides.

Fayuan Wen, Guido Caputo, Sharon Hooey, Susan Bowman, Kristine Pinkney, Peter J Krell, Basil Arif, Daniel Doucet

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fayuan WenDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Guido CaputoCanadian Forest Service, Natural Resources Canada, 1219 Queen St. East, Sault Ste Marie, ON, Canada, P6A 2E5.
Sharon HooeyCanadian Forest Service, Natural Resources Canada, 1219 Queen St. East, Sault Ste Marie, ON, Canada, P6A 2E5.
Susan BowmanCanadian Forest Service, Natural Resources Canada, 1219 Queen St. East, Sault Ste Marie, ON, Canada, P6A 2E5.
Kristine PinkneyCanadian Forest Service, Natural Resources Canada, 1219 Queen St. East, Sault Ste Marie, ON, Canada, P6A 2E5.
Peter J KrellDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Basil ArifCanadian Forest Service, Natural Resources Canada, 1219 Queen St. East, Sault Ste Marie, ON, Canada, P6A 2E5.
Daniel DoucetCanadian Forest Service, Natural Resources Canada, 1219 Queen St. East, Sault Ste Marie, ON, Canada, P6A 2E5. Dan.Doucet@NRCan.gc.ca.ORCID http://orcid.org/0000-0003-0058-255X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel cell line, NRCAN-Tb521, was developed from larvae of the longhorn beetle Tylonotus bimaculatus (Coleoptera: Cerambycidae), a pest of North American ash trees. The cell line has been successfully passaged more than 50 times and displayed very strong attachment to the substrate and a modal chromosomal count distribution of 19. Sequencing of a 649 bp fragment of the mitochondrial cytochrome oxidase I gene confirmed the identity of NRCAN-Tb521 as T. bimaculatus. The response of the cell line to 20-hydroxyecdysone and diacylhydrazine ecdysone agonist insecticides was also studied. At 10(-6) M, 20-hydroxyecdysone, tebufenozide, methoxyfenozide and halofenozide triggered the production of numerous filamentous cytoplasmic extensions, and the cells tended to form aggregates, indicative of a cell differentiation response. This response was followed by a strong decrease in viability after 4 d. Reverse transcription polymerase chain reaction (PCR) experiments and sequencing of PCR fragments showed that the 20E receptor gene EcR is expressed in the cells and that 20E, tebufenozide, methoxyfenozide and halofenozide also induce the expression of the nuclear hormone receptor gene HR3. This report establishes that NRCAN-Tb521 is a valuable in vitro model to study effects of ecdysone agonists in wood-boring cerambycids.

Indexed as

AnimalsBenzoatesCell LineCell SurvivalColeopteraDrug Evaluation, PreclinicalEcdysteroneElectron Transport Complex IVHydrazinesInsecticidesInsect ProteinsJuvenile HormonesKaryotypingReceptors, Cytoplasmic and NuclearReceptors, SteroidBenzoatesecdysone receptorEcdysteroneElectron Transport Complex IVHydrazinesInsecticidesInsect ProteinsJuvenile HormonesmethoxyfenozideN-4-chlorobenzoyl-N'-benzoyl-N'-tert-butylhydrazineReceptors, Cytoplasmic and NuclearReceptors, SteroidtebufenozideCell lineCerambycidColeopteraEcdysoneEcdysone agonist

Identifiers

PMID25952767

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.