Evidence map›Paper›PMID 25952687›Full record

Trial reportJournal of proteomics2015

Identification of differentially expressed serum proteins in gastric adenocarcinoma.

Yashwanth Subbannayya, Sartaj Ahmad Mir, Santosh Renuse, Srikanth S Manda, Sneha M Pinto, Vinuth N Puttamallesh, Hitendra Singh Solanki, H C Manju, Nazia Syed, Rakesh Sharma and 9 more

Abstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Journal of proteomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Circulating Proteins as Diagnostic Markers in Gastric Cancer.International journal of molecular sciences · 2023
    Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review
  10. Observational
  11. Article
  12. Article
  13. Review
  14. Article
  15. Effects ofTranslational cancer research · 2020
    Article
  16. Article
  17. Article
  18. Proteomic features characterization ofAllergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2019
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 6 institutions in 2 countries.

Yashwanth SubbannayyaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India; Rajiv Gandhi University of Health Sciences, Bangalore 560041, Karnataka, India; Department of Biochemistry, Kidwai Memorial Institute of Oncology, Bangalore 560029, Karnataka, India.
Sartaj Ahmad MirInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India; Manipal University, Manipal 576 104, Karnataka, India.
Santosh RenuseInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690525, India.
Srikanth S MandaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India; Centre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry 605014, India.
Sneha M PintoInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India; Manipal University, Manipal 576 104, Karnataka, India.
Vinuth N PuttamalleshInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India.
Hitendra Singh SolankiInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India.
H C ManjuInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India.
Nazia SyedInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India; Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.
Rakesh SharmaDepartment of Neurochemistry, National Institute of Mental Health and Neurosciences, Bangalore 560029, Karnataka, India.
Rita ChristopherDepartment of Neurochemistry, National Institute of Mental Health and Neurosciences, Bangalore 560029, Karnataka, India.
M VijayakumarDepartment of Surgery, Kidwai Memorial Institute of Oncology, Bangalore 560029, Karnataka, India.
K V Veerendra KumarDepartment of Surgery, Kidwai Memorial Institute of Oncology, Bangalore 560029, Karnataka, India.
T S Keshava PrasadInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India.
Girija RamaswamyDepartment of Biochemistry, Kidwai Memorial Institute of Oncology, Bangalore 560029, Karnataka, India.
Rekha V KumarDepartment of Pathology, Kidwai Memorial Institute of Oncology, Bangalore 560029, Karnataka, India.
Aditi ChatterjeeInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India.
Akhilesh PandeyMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Harsha GowdaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, India. Electronic address: harsha@ibioinformatics.org.
Institute of Bioinformatics · INKidwai Memorial Institute of Oncology · INManipal Academy of Higher Education · INNational Institute of Mental Health and Neurosciences · INPondicherry University · INJohns Hopkins University · US

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI WILLIAM George NELSON · 1985 to 2026
$208.6M
NCI NIH HHS P30 CA006973
6 · The paper itself

Abstract

Gastric adenocarcinoma is an aggressive cancer with poor prognosis. Blood based biomarkers of gastric cancer have the potential to improve diagnosis and monitoring of these tumors. Proteins that show altered levels in the circulation of gastric cancer patients could prove useful as putative biomarkers. We used an iTRAQ-based quantitative proteomic approach to identify proteins that show altered levels in the sera of patients with gastric cancer. Our study resulted in identification of 643 proteins, of which 48 proteins showed increased levels and 11 proteins showed decreased levels in serum from gastric cancer patients compared to age and sex matched healthy controls. Proteins that showed increased expression in gastric cancer included inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Mannose-binding protein C (MBL2), sex hormone-binding globulin (SHBG), insulin-like growth factor-binding protein 2 (IGFBP2), serum amyloid A protein (SAA1), Orosomucoid 1 (ORM1) and extracellular superoxide dismutase [Cu-Zn] (SOD3). We used multiple reaction monitoring assays and validated elevated levels of ITIH4 and SAA1 proteins in serum from gastric cancer patients. BIOLOGICAL SIGNIFICANCE: Gastric cancer is a highly aggressive cancer associated with high mortality. Serum-based biomarkers are of considerable interest in diagnosis and monitoring of various diseases including cancers. Gastric cancer is often diagnosed at advanced stages resulting in poor prognosis and high mortality. Pathological diagnosis using biopsy specimens remains the gold standard for diagnosis of gastric cancer. Serum-based biomarkers are of considerable importance as they are minimally invasive. In this study, we carried out quantitative proteomic profiling of serum from gastric cancer patients to identify proteins that show altered levels in gastric cancer patients. We identified more than 50 proteins that showed altered levels in gastric cancer patient sera. Validation in a large cohort of well classified patient samples would prove useful in identifying novel blood based biomarkers for gastric cancers. This article is part of a Special Issue entitled: Proteomics in India.

Indexed as

Gene Expression Regulation, NeoplasticAdenocarcinomaBiomarkers, TumorFemaleHumansMaleNeoplasm ProteinsStomach NeoplasmsBiomarkers, TumorNeoplasm ProteinsBody fluidIn vitro labelingLC-MS/MS analysisMass spectrometrySerum proteome

Identifiers

PMID25952687
PMCPMC4618463
OpenAlexW278989748

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.