ArticleJournal of industrial microbiology & biotechnology2015
Enhanced production of L-sorbose in an industrial Gluconobacter oxydans strain by identification of a strong promoter based on proteomics analysis.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 29 citations in OpenAlex.
- Transforming monosaccharides: Recent advances in rare sugar production and future exploration.BBA advances · 2025Article
- Enhanced cobalamin biosynthesis inSynthetic and systems biotechnology · 2022Article
- The industrial versatility of Gluconobacter oxydans: current applications and future perspectives.World journal of microbiology & biotechnology · 2022Review
- Enhanced production of l-sorbose by systematic engineering of dehydrogenases inSynthetic and systems biotechnology · 2022Article
- On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases.Applied microbiology and biotechnology · 2021Review
- Identification of Gradient Promoters ofFrontiers in bioengineering and biotechnology · 2021Article
- Toward fine-tuned metabolic networks in industrial microorganisms.Synthetic and systems biotechnology · 2020Article
- Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis.BMC biotechnology · 2019Article
- Efficient biosynthesis of 2-keto-D-gluconic acid by fed-batch culture of metabolically engineeredSynthetic and systems biotechnology · 2019Article
- Design of a high-efficiency synthetic system for l-asparaginase production inEngineering in life sciences · 2019Article
- Overcoming NADPH product inhibition improves D-sorbitol conversion to L-sorbose.Scientific reports · 2019Article
- RNAseq analysis of α-proteobacterium Gluconobacter oxydans 621H.BMC genomics · 2018Article
- Bicistronic expression strategy for high-level expression of recombinant proteins inEngineering in life sciences · 2017Article
- The application of powerful promoters to enhance gene expression in industrial microorganisms.World journal of microbiology & biotechnology · 2017Review
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4 authors at 2 institutions in 1 country.
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Abstract
Gluconobacter oxydans is capable of rapidly incomplete oxidation of many sugars and alcohols, which means the strain has great potential for industrial purposes. Strong promoters are one of the essential factors that can improve strain performance by overexpression of specific genes. In this study, a pipeline for screening strong promoters by proteomics analysis was established. Based on the procedure, a new strong promoter designated as P B932_2000 was identified in G. oxydans WSH-003. The promoter region was characterized based on known genome sequence information using BPROM. The strength of P B932_2000 was further assessed by analysis of enhanced green fluorescent protein (egfp) expression and comparison with egfp expression by two commonly used strong promoters, P E. coli_tufB and P G. oxydans_tufB . Both quantitative real-time PCR and fluorescence intensities for egfp gene expression showed that P B932_2000 promoter is stronger than the other two. Overexpression of D-sorbitol dehydrogenase (sldh) by P B932_2000 in G. oxydans WSH-003 enhanced the titer and productivity of L-sorbose synthesis from D-sorbitol by 12.0 % and 33.3 %, respectively. These results showed that proteomics analysis is an efficient way to identify strong promoters. The isolated promoter P B932_2000 could further facilitate the metabolic engineering of G. oxydans.
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