ArticleeLife2015
SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
87 citing papers in PubMed, 166 citations in OpenAlex.
- Obstacle bypassing and intrinsically asymmetric loop extrusion in the segment-capture model of Structural Maintenance of Chromosomes complexes.bioRxiv : the preprint server for biology · 2026Article
- Article
- Involvement of the inner surface residues of bacterial SMC protein MukB in the ssDNA binding in vitro.Communications biology · 2025Article
- Chromosome segregation dynamics during the cell cycle of Staphylococcus aureus.Nature communications · 2025Article
- Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.Cell · 2025Article
- Early onset of septal FtsK localization allows for efficient DNA segregation in SMC-deletedmBio · 2025Article
- Physical models of bacterial chromosomes.Molecular microbiology · 2025Review
- SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo.Scientific reports · 2025Article
- All eukaryotic SMC proteins induce a twist of -0.6 at each DNA loop extrusion step.Science advances · 2024Article
- Loop-extruders alter bacterial chromosome topology to direct entropic forces for segregation.Nature communications · 2024Article
- Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.Nature communications · 2024Article
- Reappraisal of the DNA phosphorothioate modification machinery: uncovering neglected functional modalities and identification of new counter-invader defense systems.Nucleic acids research · 2024Article
- Isolation and detection of DNA-protein crosslinks in mammalian cells.Nucleic acids research · 2024Article
- DNA segment capture by Smc5/6 holocomplexes.Nature structural & molecular biology · 2023Article
- Condensin pinches a short negatively supercoiled DNA loop during each round of ATP usage.The EMBO journal · 2023Article
- Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis.Nucleic acids research · 2022Article
- DNA sliding and loop formation byBiochemistry and biophysics reports · 2022Article
- Smc5/6 silences episomal transcription by a three-step function.Nature structural & molecular biology · 2022Article
- A joint-ParB interface promotes Smc DNA recruitment.Cell reports · 2022Article
- DNA tension-modulated translocation and loop extrusion by SMC complexes revealed by molecular dynamics simulations.Nucleic acids research · 2022Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Smc-ScpAB forms elongated, annular structures that promote chromosome segregation, presumably by compacting and resolving sister DNA molecules. The mechanistic basis for its action, however, is only poorly understood. Here, we have established a physical assay to determine whether the binding of condensin to native chromosomes in Bacillus subtilis involves entrapment of DNA by the Smc-ScpAB ring. To do so, we have chemically cross-linked the three ring interfaces in Smc-ScpAB and thereafter isolated intact chromosomes under protein denaturing conditions. Exclusively species of Smc-ScpA, which were previously cross-linked into covalent rings, remained associated with chromosomal DNA. DNA entrapment is abolished by mutations that interfere with the Smc ATPase cycle and strongly reduced when the recruitment factor ParB is deleted, implying that most Smc-ScpAB is loaded onto the chromosome at parS sites near the replication origin. We furthermore report a physical interaction between native Smc-ScpAB and chromosomal DNA fragments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.