ArticleScientific reports2015
Role of RNase H1 in DNA repair: removal of single ribonucleotide misincorporated into DNA in collaboration with RNase H2.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 31 citations in OpenAlex.
- Genome-wide ribonucleotide detection in Archaea.Nucleic acids research · 2025Article
- The bacterial transcription terminator, Rho, functions as an RNA:DNA hybrid (RDH) helicase in vivo.The Biochemical journal · 2025Article
- Ribonucleotide and R-Loop Damage in Plastid DNA and Mitochondrial DNA during Maize Development.Plants (Basel, Switzerland) · 2023Article
- Small Nucleolar (Sno)RNA: Therapy Lays in Translation.Non-coding RNA · 2023Article
- Strand specificity of ribonucleotide excision repair in Escherichia coli.Nucleic acids research · 2023Article
- RNA therapeutics in the clinic.Bioengineering & translational medicine · 2023Review
- The Impact of RNA-DNA Hybrids on Genome Integrity in Bacteria.Annual review of microbiology · 2022Review
- Facile preparation of model DNA interstrand cross-link repair intermediates using ribonucleotide-containing DNA.DNA repair · 2022Article
- A second hybrid-binding domain modulates the activity of Drosophila ribonuclease H1.Journal of biochemistry · 2020Article
- RNase H1 promotes replication fork progression through oppositely transcribed regions ofThe Journal of biological chemistry · 2019Article
- RNase H1 Regulates Mitochondrial Transcription and TranslationFrontiers in genetics · 2019Article
- Endogenous single-strand DNA breaks at RNA polymerase II promoters in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- Substrate Specificity for Bacterial RNases HII and HIII Is Influenced by Metal Availability.Journal of bacteriology · 2018Article
- Division of labor among Mycobacterium smegmatis RNase H enzymes: RNase H1 activity of RnhA or RnhC is essential for growth whereas RnhB and RnhA guard against killing by hydrogen peroxide in stationary phase.Nucleic acids research · 2017Article
- Genome maintenance and bioenergetics of the long-lived hypoxia-tolerant and cancer-resistant blind mole rat, Spalax: a cross-species analysis of brain transcriptome.Scientific reports · 2016Article
- The current state of eukaryotic DNA base damage and repair.Nucleic acids research · 2015Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several RNases H1 cleave the RNA-DNA junction of Okazaki fragment-like RNA-DNA/DNA substrate. This activity, termed 3'-junction ribonuclease (3'-JRNase) activity, is different from the 5'-JRNase activity of RNase H2 that cleaves the 5'-side of the ribonucleotide of the RNA-DNA junction and is required to initiate the ribonucleotide excision repair pathway. To examine whether RNase H1 exhibits 3'-JRNase activity for dsDNA containing a single ribonucleotide and can remove this ribonucleotide in collaboration with RNase H2, cleavage of a DNA8-RNA1-DNA9/DNA18 substrate with E. coli RNase H1 and H2 was analyzed. This substrate was cleaved by E. coli RNase H1 at the (5')RNA-DNA(3') junction, regardless of whether it was cleaved by E. coli RNase H2 at the (5')DNA-RNA(3') junction in advance or not. Likewise, this substrate was cleaved by E. coli RNase H2 at the (5')DNA-RNA(3') junction, regardless of whether it was cleaved by E. coli RNase H1 at the (5')RNA-DNA(3') junction in advance or not. When this substrate was cleaved by a mixture of E. coli RNases H1 and H2, the ribonucleotide was removed from the substrate. We propose that RNase H1 is involved in the excision of single ribonucleotides misincorporated into DNA in collaboration with RNase H2.
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