ArticlePloS one2015
The role of interleukin-10 and hyaluronan in murine fetal fibroblast function in vitro: implications for recapitulating fetal regenerative wound healing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds.Frontiers in bioengineering and biotechnology · 2026Article
- Review
- Eicosapentaenoic acid-rich oil supplementation activates PPAR-γ and delays skin wound healing in type 1 diabetic mice.Frontiers in immunology · 2023Article
- High-Efficient Production of Adipose-Derived Stem Cell (ADSC) Secretome Through Maturation Process and Its Non-scarring Wound Healing Applications.Frontiers in bioengineering and biotechnology · 2021Article
- High-molecular weight hyaluronan attenuates tubulointerstitial scarring in kidney injury.JCI insight · 2020Article
- The Role of the Anti-Inflammatory Cytokine Interleukin-10 in Tissue Fibrosis.Advances in wound care · 2020Review
- Emerging Role of IL-10 in Hypertrophic Scars.Frontiers in medicine · 2020Review
- The Role of an IL-10/Hyaluronan Axis in Dermal Wound Healing.Frontiers in cell and developmental biology · 2020Review
- Inflammation as an orchestrator of cutaneous scar formation: a review of the literature.Plastic and aesthetic research · 2020Article
- The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response.International journal of molecular sciences · 2019Article
- Lipid Emulsion Enriched in Omega-3 PUFA Accelerates Wound Healing: A Placebo-Controlled Animal Study.World journal of surgery · 2018Article
- Efficacy of In Vivo Electroporation-Mediated IL-10 Gene Delivery on Survival of Skin Flaps.The Journal of membrane biology · 2018Article
- Article
- Bioinformatics analysis of fibroblasts exposed to TGF‑β at the early proliferation phase of wound repair.Molecular medicine reports · 2017Article
- Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling.The Journal of clinical investigation · 2017Article
- IL-10 improves cardiac remodeling after myocardial infarction by stimulating M2 macrophage polarization and fibroblast activation.Basic research in cardiology · 2017Article
- Interleukin-10-mediated regenerative postnatal tissue repair is dependent on regulation of hyaluronan metabolismFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Regulator Versus Effector Paradigm: Interleukin-10 as Indicator of the Switching Response.Clinical reviews in allergy & immunology · 2016Review
- RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.PloS one · 2016Article
- Injury From Nematode Lung Migration Induces an IL-13-Dependent Hyaluronan Matrix.Proteoglycan researchArticle
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundMid-gestation fetal cutaneous wounds heal scarlessly and this has been attributed in part to abundant hyaluronan (HA) in the extracellular matrix (ECM) and a unique fibroblast phenotype. We recently reported a novel role for interleukin 10 (IL-10) as a regulator of HA synthesis in the fetal ECM, as well as the ability of the fetal fibroblast to produce an HA-rich pericellular matrix (PCM). We hypothesized that IL-10-mediated HA synthesis was essential to the fetal fibroblast functional phenotype and, moreover, that this phenotype could be recapitulated in adult fibroblasts via supplementation with IL-10 via an HA dependent process. METHODOLOGY/PRINCIPAL
findingsTo evaluate the differences in functional profile, we compared metabolism (MTS assay), apoptosis (caspase-3 staining), migration (scratch wound assay) and invasion (transwell assay) between C57Bl/6J murine fetal (E14.5) and adult (8 weeks) fibroblasts. We found that fetal fibroblasts have lower rates of metabolism and apoptosis, and an increased ability to migrate and invade compared to adult fibroblasts, and that these effects were dependent on IL-10 and HA synthase activity. Further, addition of IL-10 to adult fibroblasts resulted in increased fibroblast migration and invasion and recapitulated the fetal phenotype in an HA-dependent manner. CONCLUSIONS/SIGNIFICANCE: Our data demonstrates the functional differences between fetal and adult fibroblasts, and that IL-10 mediated HA synthesis is essential for the fetal fibroblasts' enhanced invasion and migration properties. Moreover, IL-10 via an HA-dependent mechanism can recapitulate this aspect of the fetal phenotype in adult fibroblasts, suggesting a novel mechanism of IL-10 in regenerative wound healing.
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