ArticlePloS one2015
piRNAs from Pig Testis Provide Evidence for a Conserved Role of the Piwi Pathway in Post-Transcriptional Gene Regulation in Mammals.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- piRNAs as Potential Regulators of Mammary Gland Development and Pathology in Livestock.Veterinary sciences · 2025Review
- Young SINEs in pig genomes impact gene regulation, genetic diversity, and complex traits.Communications biology · 2023Article
- cDNA Cloning of Feline PIWIL1 and Evaluation of Expression in the Testis of the Domestic Cat.International journal of molecular sciences · 2023Article
- Effects of hsa-piR-32877 Suppression with Antisense LNA GapmeRs on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells.International journal of molecular and cellular medicine · 2023Article
- Identification and Characterization of Polymorphisms in piRNA Regions.Current issues in molecular biology · 2022Article
- piRNAclusterDB 2.0: update and expansion of the piRNA cluster database.Nucleic acids research · 2022Article
- Characteristics of piRNAs and their comparative profiling in testes of sheep with different fertility.Frontiers in genetics · 2022Article
- A Review of Discovery Profiling of PIWI-Interacting RNAs and Their Diverse Functions in Metazoans.International journal of molecular sciences · 2021Review
- Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs.Genome biology · 2021Review
- Long first exons and epigenetic marks distinguish conserved pachytene piRNA clusters from other mammalian genes.Nature communications · 2021Article
- 5' tRNA halves are highly expressed in the primate hippocampus and might sequence-specifically regulate gene expression.RNA (New York, N.Y.) · 2020Article
- Primate piRNA Cluster Evolution Suggests Limited Relevance of Pseudogenes in piRNA-Mediated Gene Regulation.Genome biology and evolution · 2019Article
- Identification of piRNAs and piRNA clusters in the testes of the Mongolian horse.Scientific reports · 2019Article
- Non-coding RNAs predict recurrence-free survival of patients with hypoxic tumours.Scientific reports · 2018Article
- Article
- Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis.RNA biology · 2017Article
- Two modes of targeting transposable elements by piRNA pathway in human testis.RNA (New York, N.Y.) · 2017Article
- Protein-Coding Genes' Retrocopies and Their Functions.Viruses · 2017Review
- Oncogenomic disruptions in arsenic-induced carcinogenesis.Oncotarget · 2017Review
- Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.Molecular and cellular biochemistry · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Piwi-interacting (pi-) RNAs guide germline-expressed Piwi proteins in order to suppress the activity of transposable elements (TEs). But notably, the majority of pachytene piRNAs in mammalian testes is not related to TEs. This raises the question of whether the Piwi/piRNA pathway exerts functions beyond TE silencing. Although gene-derived piRNAs were described many times, a possible gene-regulatory function was doubted due to the absence of antisense piRNAs. Here we sequenced and analyzed piRNAs expressed in the adult testis of the pig, as this taxon possesses the full set of mammalian Piwi paralogs while their spermatozoa are marked by an extreme fitness due to selective breeding. We provide an exhaustive characterization of porcine piRNAs and genomic piRNA clusters. Moreover, we reveal that both sense and antisense piRNAs derive from protein-coding genes, while exhibiting features that clearly show that they originate from the Piwi/piRNA-mediated post-transcriptional silencing pathway, commonly referred to as ping-pong cycle. We further show that the majority of identified piRNA clusters in the porcine genome spans exonic sequences of protein-coding genes or pseudogenes, which reveals a mechanism by which primary antisense piRNAs directed against mRNA can be generated. Our data provide evidence that spliced mRNAs, derived from such loci, are not only targeted by piRNAs but are also subject to ping-pong cycle processing. Finally, we demonstrate that homologous genes are targeted and processed by piRNAs in pig, mouse and human. Altogether, this strongly suggests a conserved role for the mammalian Piwi/piRNA pathway in post-transcriptional regulation of protein-coding genes, which did not receive much attention so far.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.