ArticleeLife2015
Visualization and functional dissection of coaxial paired SpoIIIE channels across the sporulation septum.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis.Communications biology · 2026Article
- Deep-learning deconvolution and segmentation of fluorescent membranes for high-precision bacterial cell-size profiling.Communications biology · 2026Article
- Catabolism of germinant amino acids is required to prevent premature spore germination inmBio · 2024Article
- A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis.MicrobiologyOpen · 2023Article
- Chromosome remodelling by SMC/Condensin inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.Developmental cell · 2021Article
- Metabolic differentiation and intercellular nurturing underpin bacterial endospore formation.Science advances · 2021Article
- Milestones inMicrobial cell (Graz, Austria) · 2020Review
- Cohesion of Sister Chromosome Termini during the Early Stages of Sporulation in Bacillus subtilis.Journal of bacteriology · 2020Article
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy.International journal of molecular sciences · 2019Article
- The molecular architecture of engulfment duringeLife · 2019Article
- Single-Molecule Tracking of DNA Translocases in Bacillus subtilis Reveals Strikingly Different Dynamics of SftA, SpoIIIE, and FtsA.Applied and environmental microbiology · 2018Article
- Spatiotemporally regulated proteolysis to dissect the role of vegetative proteins during Bacillus subtilis sporulation: cell-specific requirement of σMolecular microbiology · 2018Article
- Mechanochemical coupling and bi-phasic force-velocity dependence in the ultra-fast ring ATPase SpoIIIE.eLife · 2018Article
- Article
- Identification of the S-transferase like superfamily bacillithiol transferases encoded by Bacillus subtilis.PloS one · 2018Article
- Unraveling the Thousand Word Picture: An Introduction to Super-Resolution Data Analysis.Chemical reviews · 2017Article
- Super-resolution microscopy with DNA-PAINT.Nature protocols · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
SpoIIIE is a membrane-anchored DNA translocase that localizes to the septal midpoint to mediate chromosome translocation and membrane fission during Bacillus subtilis sporulation. Here we use cell-specific protein degradation and quantitative photoactivated localization microscopy in strains with a thick sporulation septum to investigate the architecture and function of the SpoIIIE DNA translocation complex in vivo. We were able to visualize SpoIIIE complexes with approximately equal numbers of molecules in the mother cell and the forespore. Cell-specific protein degradation showed that only the mother cell complex is required to translocate DNA into the forespore, whereas degradation in either cell reverses membrane fission. Our data suggest that SpoIIIE assembles a coaxially paired channel for each chromosome arm comprised of one hexamer in each cell to maintain membrane fission during DNA translocation. We show that SpoIIIE can operate, in principle, as a bi-directional motor that exports DNA.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.