ArticleProteome science2015
Comparative proteomics of Bt-transgenic and non-transgenic cotton leaves.
Article in Proteome science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
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- Biotechnology and Solutions: Insect-Pest-Resistance Management for Improvement and Development of Bt Cotton (Plants (Basel, Switzerland) · 2023Article
- Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot.BMC plant biology · 2023Article
- Proteomic Analysis ofPlants (Basel, Switzerland) · 2023Article
- Proteomic and Metabolomic Evaluation of Insect- and Herbicide-Resistant Maize Seeds.Metabolites · 2022Article
- Comparative proteome analyses of rhizomania resistant transgenic sugar beets based on RNA silencing mechanism.GM crops & food · 2021Article
- Integrated proteomics and metabolomics analysis of transgenic and gene-stacked maize line seeds.GM crops & food · 2021Article
- Morpho-Physiological and Proteomic Response of Bt-Cotton and Non-Bt Cotton to Drought Stress.Frontiers in plant science · 2021Article
- Untargeted Proteomics-Based Approach to Investigate Unintended Changes in Genetically Modified Maize for Environmental Risk Assessment Purpose.Frontiers in toxicology · 2021Article
- Evaluating the effect ofJournal of biological research (Thessalonike, Greece) · 2020Article
- CRISPR/Cas9 Directed Mutagenesis ofInternational journal of molecular sciences · 2020Article
- Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island.PloS one · 2020Article
- Comparative Proteomic Analysis of Molecular Differences between Leaves of Wild-Type Upland Cotton and ItsMolecules (Basel, Switzerland) · 2019Article
- An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS.BMC genomics · 2019Article
- Carbon and nitrogen partitioning of transgenic rice T2A-1 (Cry2A*) with different nitrogen treatments.Scientific reports · 2019Article
- Article
- iTRAQ-based quantitative tissue proteomic analysis of differentially expressed proteins (DEPs) in non-transgenic and transgenic soybean seeds.Scientific reports · 2018Article
- Reply to 'Comments on two recent publications on GM maize and Roundup'.Scientific reports · 2018Article
- Prospecting for Microelement Function and Biosafety Assessment of Transgenic Cereal Plants.Frontiers in plant science · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs the rapid growth of the commercialized acreage in genetically modified (GM) crops, the unintended effects of GM crops' biosafety assessment have been given much attention. To investigate whether transgenic events cause unintended effects, comparative proteomics of cotton leaves between the commercial transgenic Bt + CpTI cotton SGK321 (BT) clone and its non-transgenic parental counterpart SY321 wild type (WT) was performed.
resultsUsing enzyme linked immunosorbent assay (ELISA), Cry1Ac toxin protein was detected in the BT leaves, while its content was only 0.31 pg/g. By 2-DE, 58 differentially expressed proteins (DEPs) were detected. Among them 35 were identified by MS. These identified DEPs were mainly involved in carbohydrate transport and metabolism, chaperones related to post-translational modification and energy production. Pathway analysis revealed that most of the DEPs were implicated in carbon fixation and photosynthesis, glyoxylate and dicarboxylate metabolism, and oxidative pentose phosphate pathway. Thirteen identified proteins were involved in protein-protein interaction. The protein interactions were mainly involved in photosynthesis and energy metabolite pathway.
conclusionsOur study demonstrated that exogenous DNA in a host cotton genome can affect the plant growth and photosynthesis. Although some unintended variations of proteins were found between BT and WT cotton, no toxic proteins or allergens were detected. This study verified genetically modified operation did not sharply alter cotton leaf proteome, and the target proteins were hardly checked by traditional proteomic analysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.