ArticleProceedings of the National Academy of Sciences of the United States of America1989
Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 116 citations in OpenAlex.
- Structural basis of secreted acid phosphatase polymerization in the Leishmania parasite.Protein science : a publication of the Protein Society · 2026Article
- LeishIF3d is a non-canonical cap-binding protein inFrontiers in molecular biosciences · 2023Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Characterization of an Atypical eIF4E Ortholog inInternational journal of molecular sciences · 2021Article
- LeishIF4E-5 Is a Promastigote-Specific Cap-Binding Protein inInternational journal of molecular sciences · 2021Article
- Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated hemizygous deletion.PLoS neglected tropical diseases · 2021Article
- Article
- Deletion of a Single LeishIF4E-3 Allele by the CRISPR-Cas9 System Alters Cell Morphology and Infectivity ofmSphere · 2019Article
- Nutritional stress targets LeishIF4E-3 to storage granules that contain RNA and ribosome components in Leishmania.PLoS neglected tropical diseases · 2019Article
- Hsp90 inhibitors radicicol and geldanamycin have opposing effects on Leishmania Aha1-dependent proliferation.Cell stress & chaperones · 2017Article
- A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum.Antimicrobial agents and chemotherapy · 2016Article
- Leishmania donovani P23 protects parasites against HSP90 inhibitor-mediated growth arrest.Cell stress & chaperones · 2015Article
- A small heat shock protein is essential for thermotolerance and intracellular survival of Leishmania donovani.Journal of cell science · 2014Article
- A novel marker, ARM58, confers antimony resistance to Leishmania spp.International journal for parasitology. Drugs and drug resistance · 2014Article
- Reduced antimony accumulation in ARM58-overexpressing Leishmania infantum.Antimicrobial agents and chemotherapy · 2014Article
- The Hsp90-Sti1 interaction is critical for Leishmania donovani proliferation in both life cycle stages.Cellular microbiology · 2013Article
- A novel 4E-interacting protein in Leishmania is involved in stage-specific translation pathways.Nucleic acids research · 2011Article
- The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability.Cell stress & chaperones · 2010Article
- Preferential translation of Hsp83 in Leishmania requires a thermosensitive polypyrimidine-rich element in the 3' UTR and involves scanning of the 5' UTR.RNA (New York, N.Y.) · 2010Article
- Evolutionary changes in the Leishmania eIF4F complex involve variations in the eIF4E-eIF4G interactions.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.
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