ArticleMolecular biology of the cell2015
A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Altered methylation pattern in EXOC4 is associated with stroke outcome: an epigenome-wide association study.Clinical epigenetics · 2022Pooled it
- The origin of septin ring size control in budding yeast.The EMBO journal · 2025Article
- The roles of yeast formins and their regulators Bud6 and Bil2 in the pheromone response.Molecular biology of the cell · 2024Article
- The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium.Communications biology · 2024Article
- Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane.Frontiers in molecular biosciences · 2024Review
- Article
- Rice STOMATAL CYTOKINESIS DEFECTIVE2 regulates cell expansion by affecting vesicular trafficking in rice.Plant physiology · 2022Article
- Cross-talk between clathrin-dependent post-Golgi trafficking and clathrin-mediated endocytosis in Arabidopsis root cells.The Plant cell · 2021Article
- Avidity-driven polarity establishment via multivalent lipid-GTPase module interactions.The EMBO journal · 2019Article
- Evolution of late steps in exocytosis: conservation and specialization of the exocyst complex.Wellcome open research · 2019Article
- Recent advances in understandingF1000Research · 2019Review
- Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion.Molecular biology of the cell · 2018Article
- Regulation of Cell Polarity by Exocyst-Mediated Trafficking.Cold Spring Harbor perspectives in biology · 2018Review
- Cytoplasmic cleavage of DPPA3 is required for intracellular trafficking and cleavage-stage development in mice.Nature communications · 2017Article
- Distinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast.Molecular biology of the cell · 2017Article
- SCD1 and SCD2 Form a Complex That Functions with the Exocyst and RabE1 in Exocytosis and Cytokinesis.The Plant cell · 2017Article
- MoEnd3 regulates appressorium formation and virulence through mediating endocytosis in rice blast fungus Magnaporthe oryzae.PLoS pathogens · 2017Article
- Article
- Analysis of Exocyst Subunit EXO70 Family Reveals Distinct Membrane Polar Domains in Tobacco Pollen Tubes.Plant physiology · 2017Article
- Role of Arf GTPases in fungal morphogenesis and virulence.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis.
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