ArticleTransgenic research2015
Transgenic plants over-expressing insect-specific microRNA acquire insecticidal activity against Helicoverpa armigera: an alternative to Bt-toxin technology.
Article in Transgenic research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- A Comprehensive Larval microRNA Atlas ofInternational journal of molecular sciences · 2026Article
- Review
- The Role of MicroRNA-Based Strategies in Optimizing Plant Biomass Composition for Bio-Based Packaging Materials.Plants (Basel, Switzerland) · 2025Review
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- SilencingInsects · 2024Article
- Molecular Characterization of miRNAs inBiology · 2024Article
- Research Progress on miRNAs and Artificial miRNAs in Insect and Disease Resistance and Breeding in Plants.Genes · 2024Review
- microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms.International journal of molecular sciences · 2024Review
- Neutral Dietary Effects of Two MicroRNAs, Csu-Novel-260 and Csu-Mir-14, on the Non-Target ArthropodPlants (Basel, Switzerland) · 2023Article
- Glutamate-GABA imbalance mediated by miR-8-5p and its STTM regulates phase-related behavior of locusts.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Guiding RNAi Design Through Characterization of Endogenous Small RNA Pathways.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Recent advances and potential applications of cross-kingdom movement of miRNAs in modulating plant's disease response.RNA biology · 2022Review
- Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development.International journal of molecular sciences · 2021Article
- Spatio-Temporal Profiling ofJournal of fungi (Basel, Switzerland) · 2021Article
- Dissecting protein domain variability in the core RNA interference machinery of five insect orders.RNA biology · 2021Article
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Article
- Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata.Plant biotechnology journal · 2021Article
- MicroRNAs: Potential Targets for Developing Stress-Tolerant Crops.Life (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The success of Bt transgenics in controlling predation of crops has been tempered by sporadic emergence of resistance in targeted insect larvae. Such emerging threats have prompted the search for novel insecticidal molecules that are specific and could be expressed through plants. We have resorted to small RNA-based technology for an investigative search and focused our attention to an insect-specific miRNA that interferes with the insect molting process resulting in the death of the larvae. In this study, we report the designing of a vector that produces artificial microRNA (amiR), namely amiR-24, which targets the chitinase gene of Helicoverpa armigera. This vector was used as transgene in tobacco. Northern blot and real-time analysis revealed the high level expression of amiR-24 in transgenic tobacco plants. Larvae feeding on the transgenic plants ceased to molt further and eventually died. Our results demonstrate that transgenic tobacco plants can express amiR-24 insectice specific to H. armigera.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.