ArticleTransgenic research2015
Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1.
Article in Transgenic research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- Silencing of disease susceptibility genes: an effective disease resistance strategy against fungal pathogens.Plant cell reports · 2025Review
- Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.Molecular plant pathology · 2025Article
- Recent Developments in CRISPR/Cas9 Genome-Editing Technology Related to Plant Disease Resistance and Abiotic Stress Tolerance.Biology · 2023Review
- Genome engineering of disease susceptibility genes for enhancing resistance in plants.Functional & integrative genomics · 2023Review
- Genomic Analysis Highlights Putative Defective Susceptibility Genes in Tomato Germplasm.Plants (Basel, Switzerland) · 2023Article
- Inactivation of tomatoFrontiers in plant science · 2023Article
- Genome-wide identification, evolution, and expression analysis ofFrontiers in plant science · 2023Article
- The origin and distribution of 'Kokubu'-type splice-site mutations of theBreeding science · 2022Article
- Evolution of the MLO gene families in octoploid strawberry (Fragaria ×ananassa) and progenitor diploid species identified potential genes for strawberry powdery mildew resistance.Horticulture research · 2021Article
- Identification of Susceptibility Genes inPlants (Basel, Switzerland) · 2021Article
- Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes.Scientific reports · 2021Article
- Genome-Wide Characterization of theFrontiers in plant science · 2021Article
- Screening of CRISPR/Cas9 gRNA for mimicking Powdery Mildew resistant MLO ol-2 mutant.Bioinformation · 2021Article
- DNA marker development by the allele-specific detection of powdery mildew resistance loci derived from Japanese domestic tobacco cultivar 'Kokubu'.Breeding science · 2020Article
- Genome wide characterization revealed MnMLO2 and MnMLO6A as candidate genes involved in powdery mildew susceptibility in mulberry.Molecular biology reports · 2020Article
- Ty-1, a universal resistance gene against geminiviruses that is compromised by co-replication of a betasatellite.Molecular plant pathology · 2020Article
- Arabidopsis mlo3 mutant plants exhibit spontaneous callose deposition and signs of early leaf senescence.Plant molecular biology · 2019Article
- Comparative genome analyses reveal sequence features reflecting distinct modes of host-adaptation between dicot and monocot powdery mildew.BMC genomics · 2018Article
- Article
- Genome-wide sequence variations between wild and cultivated tomato species revisited by whole genome sequence mapping.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specific homologs of the plant Mildew Locus O (MLO) gene family act as susceptibility factors towards the powdery mildew (PM) fungal disease, causing significant economic losses in agricultural settings. Thus, in order to obtain PM resistant phenotypes, a general breeding strategy has been proposed, based on the selective inactivation of MLO susceptibility genes across cultivated species. In this study, PCR-based methodologies were used in order to isolate MLO genes from cultivated solanaceous crops that are hosts for PM fungi, namely eggplant, potato and tobacco, which were named SmMLO1, StMLO1 and NtMLO1, respectively. Based on phylogenetic analysis and sequence alignment, these genes were predicted to be orthologs of tomato SlMLO1 and pepper CaMLO2, previously shown to be required for PM pathogenesis. Full-length sequence of the tobacco homolog NtMLO1 was used for a heterologous transgenic complementation assay, resulting in its characterization as a PM susceptibility gene. The same assay showed that a single nucleotide change in a mutated NtMLO1 allele leads to complete gene loss-of-function. Results here presented, also including a complete overview of the tobacco and potato MLO gene families, are valuable to study MLO gene evolution in Solanaceae and for molecular breeding approaches aimed at introducing PM resistance using strategies of reverse genetics.
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