Evidence map›Paper›PMID 25946192›Full record

ArticlePloS one2015

CRISPR Diversity in E. coli Isolates from Australian Animals, Humans and Environmental Waters.

Maxim S Sheludchenko, Flavia Huygens, Helen Stratton, Megan Hargreaves

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Maxim S SheludchenkoSmart Water Research Centre, Griffith University, Southport, Queensland, Australia; University of the Sunshine Coast, Sippy Downs, Queensland, Australia.
Flavia HuygensInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
Helen StrattonSmart Water Research Centre, Griffith University, Southport, Queensland, Australia.
Megan HargreavesInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Seventy four SNP genotypes and 54 E. coli genomes from kangaroo, Tasmanian devil, reptile, cattle, dog, horse, duck, bird, fish, rodent, human and environmental water sources were screened for the presence of the CRISPR 2.1 loci flanked by cas2 and iap genes. CRISPR 2.1 regions were found in 49% of the strains analysed. The majority of human E. coli isolates lacked the CRISPR 2.1 locus. We described 76 CRISPR 2.1 positive isolates originating from Australian animals and humans, which contained a total of 764 spacer sequences. CRISPR arrays demonstrated a long history of phage attacks especially in isolates from birds (up to 40 spacers). The most prevalent spacer (1.6%) was an ancient spacer found mainly in human, horse, duck, rodent, reptile and environmental water sources. The sequence of this spacer matched the intestinal P7 phage and the pO111 plasmid of E. coli.

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsPolymorphism, Single NucleotideAnimalsAustraliaCattleDNA, IntergenicDogsEndonucleasesEscherichia coliEscherichia coli ProteinsHumansSoil MicrobiologyWater MicrobiologyCas2 protein, E coliDNA, IntergenicEndonucleasesEscherichia coli Proteinsiap protein, E coli

Identifiers

PMID25946192
PMCPMC4422515

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.