ArticleJournal of microscopy2015
Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser.
Article in Journal of microscopy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- High-resolution 3D fluorescent imaging of intact tissues.International journal of cardiology and cardiovascular diseases · 2021Article
- Depth-resolved Mueller matrix polarimetry microscopy of the rat cornea.Biomedical optics express · 2020Article
- Multiplex protein-specific microscopy with ultraviolet surface excitation.Biomedical optics express · 2020Article
- Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.Brain structure & function · 2018Review
- Visible continuum pulses based on enhanced dispersive wave generation for endogenous fluorescence imaging.Biomedical optics express · 2017Article
- Effects of laser polarization on responses of the fluorescent CaNeurophotonics · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have studied the wavelength dependence of the two-photon excitation efficiency for a number of common UV excitable fluorescent dyes; the nuclear stains DAPI, Hoechst and SYTOX Green, chitin- and cellulose-staining dye Calcofluor White and Alexa Fluor 350, in the visible and near-infrared wavelength range (540-800 nm). For several of the dyes, we observe a substantial increase in the fluorescence emission intensity for shorter excitation wavelengths than the 680 nm which is the shortest wavelength usually available for two-photon microscopy. We also find that although the rate of photo-bleaching increases at shorter wavelengths, it is still possible to acquire many images with higher fluorescence intensity. This is particularly useful for applications where the aim is to image the structure, rather than monitoring changes in emission intensity over extended periods of time. We measure the excitation spectrum when the dyes are used to stain biological specimens to get a more accurate representation of the spectrum of the dye in a cell environment as compared to solution-based measurements.
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Registered trials
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