ArticleIranian journal of basic medical sciences2015
Nerve growth factor in human semen: Effect of nerve growth factor on the normozoospermic men during cryopreservation process.
Article in Iranian journal of basic medical sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06560216 (Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm), which is not on this map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Platelet-rich plasma (PRP) and the future of male fertility: a path forward for personalized and regenerative therapies.Stem cell research & therapy · 2025Pooled it
- Molecular Advances in Male Infertility and Fertility: Importance of Redox Regulation and Oxidative Stress.International journal of molecular sciences · 2026Review
- Environmental influences on seminal plasma: Molecular and functional insights.The Journal of reproduction and development · 2026Review
- Effect of Recombinant NGF Encapsulated in Chitosan on Rabbit Sperm Traits and Main Metabolic Pathways.Biology · 2025Article
- NGF, EPO, and IGF-1 in the Male Reproductive System.Journal of clinical medicine · 2024Review
- A Possible Role for Nerve Growth Factor and Its Receptors in Human Sperm Pathology.Biomedicines · 2023Article
- NGF and the Male Reproductive System: Potential Clinical Applications in Infertility.International journal of molecular sciences · 2022Review
- The role of growth factors in human sperm parameters: A review of in vitro studies.International journal of reproductive biomedicine · 2022Review
- Histopathologic evaluation of the inflammatory factors and stromal cells in the endometriosis lesions: A case-control study.International journal of reproductive biomedicine · 2022Article
- Expression of Nerve Growth Factor and Its Receptor TrkA in the Reproductive System of Adult Zebrafish.Veterinary sciences · 2022Article
- Serum Nerve Growth Factor Levels as a Predictor of Bull Candidate Semen Quality of Madura Cattle.Veterinary medicine international · 2022Article
- Nerve Growth Factor (NGF) and Animal Reproduction.Advances in experimental medicine and biology · 2021Review
- Effects of NGF Addition on Llama (Frontiers in veterinary science · 2020Article
- Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome:International journal of stem cells · 2019Article
- In vitro effect of nerve growth factor on the main traits of rabbit sperm.Reproductive biology and endocrinology : RB&E · 2019Article
- Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits.PloS one · 2019Article
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- Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation.Reproductive biology and endocrinology : RB&E · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesAlthough routinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of spermatozoa improved after addition of supplementation of antioxidant molecules to freezing media. Nerve growth factor (NGF) as one of the prosurvival substances has gained great attention in recent years. The aim of this study was the usage of NGF as prosurvival factor after cryopreservation process of human semen samples to assess the motility and viability of sperm, nitric oxide (NO) concentration, and DNA fragmentation in normozoospermic men. MATERIALS AND
methodsSemen samples were collected from 25 normozoospermic men and were divided into fresh semen samples as control group, frozen-thawed semen samples without addition of exogenous NGF, and three groups of semen samples cryopreserved with addition of exogenous NGF (0.5, 1, and 5 ng/ml) in freezing medium. Viability was assessed by eosin-negrosin staining technique. Motility was evaluated with inverted microscope. NO concentration and apoptosis content were measured with flow cytometry.
resultsResults showed that exogenous NGF at 0.5 ng/ml could significantly (P-value <0.05) influence viability, motility, nitric oxide, and DNA fragmentation content.
conclusionExogenous NGF as cryoprotectant improved sperm viability and motility, increased intracellular NO concentration, and decreased apoptosis content in normal human spermatozoa.
Indexed as
Identifiers
25945243PMC4414996W1911890240What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.