Evidence map›Paper›PMID 25945151›Full record

ArticleOxidative medicine and cellular longevity2015

Smooth muscle specific overexpression of p22phox potentiates carotid artery wall thickening in response to injury.

Michael R Manogue, Justin R Bennett, Drury S Holland, Chung-Sik Choi, Douglas A Drake, Mark S Taylor, David S Weber

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oxidative medicine and cellular longevity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Changes in vascular reactivity and endothelial CaMicrocirculation (New York, N.Y. : 1994) · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Michael R ManogueDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
Justin R BennettDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
Drury S HollandDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
Chung-Sik ChoiDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
Douglas A DrakeDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
Mark S TaylorDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
David S WeberDepartment of Physiology, University of South Alabama, Mobile, AL 36688, USA.
University of South Alabama · US

Funding

Reactive Oxygen Species Regulation of Vascular Smooth Muscle Cell MigrationR01HL084159 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI WEBER, DAVID S · 2008 to 2012
$1.6M
NHLBI NIH HHS R01 HL084159
6 · The paper itself

Abstract

We hypothesized that transgenic mice overexpressing the p22(phox) subunit of the NADPH oxidase selectively in smooth muscle (Tg(p22smc)) would exhibit an exacerbated response to transluminal carotid injury compared to wild-type mice. To examine the role of reactive oxygen species (ROS) as a mediator of vascular injury, the injury response was quantified by measuring wall thickness (WT) and cross-sectional wall area (CSWA) of the injured and noninjured arteries in both Tg(p22smc) and wild-type animals at days 3, 7, and 14 after injury. Akt, p38 MAPK, and Src activation were evaluated at the same time points using Western blotting. WT and CSWA following injury were significantly greater in Tg(p22smc) mice at both 7 and 14 days after injury while noninjured contralateral carotids were similar between groups. Apocynin treatment attenuated the injury response in both groups and rendered the response similar between Tg(p22smc) mice and wild-type mice. Following injury, carotid arteries from Tg(p22smc) mice demonstrated elevated activation of Akt at day 3, while p38 MAPK and Src activation was elevated at day 7 compared to wild-type mice. Both increased activation and temporal regulation of these signaling pathways may contribute to enhanced vascular growth in response to injury in this transgenic model of elevated vascular ROS.

Indexed as

AnimalsCarotid ArteriesCarotid Artery InjuriesCytochrome b GroupMiceMice, Inbred C57BLMice, TransgenicMuscle, Smooth, VascularNADPH Oxidasesp38 Mitogen-Activated Protein KinasesPhosphorylationProto-Oncogene Proteins c-aktReactive Oxygen SpeciesSignal Transductionsrc-Family KinasesCyba protein, mouseCytochrome b GroupNADPH Oxidasesp38 Mitogen-Activated Protein KinasesProto-Oncogene Proteins c-aktReactive Oxygen Speciessrc-Family Kinases

Identifiers

PMID25945151
PMCPMC4402189
OpenAlexW2038050915

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.