Evidence map›Paper›PMID 25944824›Full record

ArticlePlant physiology2015

Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.

Jörg Schwender, Inga Hebbelmann, Nicolas Heinzel, Tatjana Hildebrandt, Alistair Rogers, Dhiraj Naik, Matthias Klapperstück, Hans-Peter Braun, Falk Schreiber, Peter Denolf and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed, 1 pooled it
6.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 1 synthesis or guideline pooled it, 102 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Oil as a Hindrance to Oat (Foods (Basel, Switzerland) · 2026
    Review
  4. Review
  5. Non-canonical plant metabolism.Nature plants · 2025
    Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. An expanded role for the transcription factorFrontiers in plant science · 2022
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 5 countries.

Jörg SchwenderBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Inga HebbelmannBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Nicolas HeinzelBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Tatjana HildebrandtBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Alistair RogersBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Dhiraj NaikBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).ORCID http://orcid.org/0000-0002-1226-2337
Matthias KlapperstückBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Hans-Peter BraunBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).ORCID http://orcid.org/0000-0002-4459-9727
Falk SchreiberBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Peter DenolfBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).ORCID http://orcid.org/0000-0003-4567-2700
Ljudmilla BorisjukBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.).
Hardy RolletschekBrookhaven National Laboratory, Biological, Environmental, and Climate Sciences Department, Upton, New York 11973 (J.S., I.H., A.R., D.N.);Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany (N.H., L.B., H.R.);Institut für Pflanzengenetik, Universität Hannover, 30419 Hannover, Germany (T.H., H.-P.B.);Department of Environmental Science, Indian Institute of Advanced Research, Koba, Gandhinagar 382007, Gujarat, India (D.N.);Clayton School of Information Technology, Monash University, Melbourne, Victoria 3800, Australia (M.K., F.S.);Institute of Computer Science, University Halle-Wittenberg, 06120 Halle, Germany (F.S.); andBayer CropScience, 9052 Zwijnaarde, Belgium (P.D.) rollet@ipk-gatersleben.de.
Brookhaven National Laboratory · USLeibniz Association · DELeibniz University Hannover · DEBayer (Belgium) · BEIndian Institute of Advanced Research · INLeibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) · DEWittenberg University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. Overall, we observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Quantitative data were also used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism.

Indexed as

Multilevel AnalysisAmino AcidsBiocatalysisBiomassBrassica napusCarbohydrate MetabolismCarbonChromatography, LiquidGlycolysisLipid MetabolismMass SpectrometryMetabolic Flux AnalysisModels, BiologicalPlant OilsPlant ProteinsProteomeAmino AcidsCarbonPlant OilsPlant ProteinsProteomeStarch

Identifiers

PMID25944824
PMCPMC4741336
OpenAlexW1834965185

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.