ArticlePlant molecular biology2015
DNA METHYLTRANSFERASE 1 is involved in (m)CG and (m)CCG DNA methylation and is essential for sporophyte development in Physcomitrella patens.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Evolution of Epigenetic Regulation in Plant Reproduction.Epigenomes · 2026Review
- Structure and autoinhibitory regulation of MET1 in the maintenance of plant CG methylation.The Plant cell · 2025Article
- Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducingGenome research · 2025Article
- Transcriptional Silencing of 35S rDNA inInternational journal of molecular sciences · 2024Article
- A complex network of interactions governs DNA methylation at telomeric regions.Nucleic acids research · 2022Article
- Multi-omics data integration reveals link between epigenetic modifications and gene expression in sugar beet (Beta vulgaris subsp. vulgaris) in response to cold.BMC genomics · 2022Article
- Structure and Mechanism of Plant DNA Methyltransferases.Advances in experimental medicine and biology · 2022Article
- The epigenetic origin of life history transitions in plants and algae.Plant reproduction · 2021Review
- Evolution of CG Methylation Maintenance Machinery in Plants.Epigenomes · 2021Review
- DNA repair- and nucleotide metabolism-related genes exhibit differential CHG methylation patterns in natural and synthetic polyploids (Brassica napus L.).Horticulture research · 2021Article
- Epigenetic reprogramming rewires transcription during the alternation of generations in Arabidopsis.eLife · 2021Article
- DNA methylation mutants inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Characterisation of evolutionarily conserved key players affecting eukaryotic flagellar motility and fertility using a moss model.The New phytologist · 2020Article
- RdDM-independent de novo and heterochromatin DNA methylation by plant CMT and DNMT3 orthologs.Nature communications · 2019Article
- Unique Epigenetic Features of Ribosomal RNA Genes (rDNA) in Early Diverging Plants (Bryophytes).Frontiers in plant science · 2019Article
- Mechanistic insights into plant SUVH family H3K9 methyltransferases and their binding to context-biased non-CG DNA methylation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- DNA Methylation and the Evolution of Developmental Complexity in Plants.Frontiers in plant science · 2018Review
- Cytosine methylation at CpCpG sites triggers accumulation of non-CpG methylation in gene bodies.Nucleic acids research · 2017Article
- Inter-individual variation in DNA methylation is largely restricted to tissue-specific differentially methylated regions in maize.BMC plant biology · 2017Article
- Epigenetic Control of Phenotypic Plasticity in the Filamentous Fungus Neurospora crassa.G3 (Bethesda, Md.) · 2016Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation has a crucial role in plant development regulating gene expression and silencing of transposable elements. Maintenance DNA methylation in plants occurs at symmetrical (m)CG and (m)CHG contexts ((m) = methylated) and is maintained by DNA METHYLTRANSFERASE 1 (MET1) and CHROMOMETHYLASE (CMT) DNA methyltransferase protein families, respectively. While angiosperm genomes encode for several members of MET1 and CMT families, the moss Physcomitrella patens, serving as a model for early divergent land plants, carries a single member of each family. To determine the function of P. patens PpMET we generated ΔPpmet deletion mutant which lost (m)CG and unexpectedly (m)CCG methylation at loci tested. In order to evaluate the extent of (m)CCG methylation by MET1, we reexamined the Arabidopsis thaliana Atmet1 mutant methylome and found a similar pattern of methylation loss, suggesting that maintenance of DNA methylation by MET1 is conserved through land plant evolution. While ΔPpmet displayed no phenotypic alterations during its gametophytic phase, it failed to develop sporophytes, indicating that PpMET plays a role in gametogenesis or early sporophyte development. Expression array analysis revealed that the deletion of PpMET resulted in upregulation of two genes and multiple repetitive sequences. In parallel, expression analysis of the previously reported ΔPpcmt mutant showed that lack of PpCMT triggers overexpression of genes. This overexpression combined with loss of (m)CHG and its pleiotropic phenotype, implies that PpCMT has an essential evolutionary conserved role in the epigenetic control of gene expression. Collectively, our results suggest functional conservation of MET1 and CMT families during land plant evolution. A model describing the relationship between MET1 and CMT in CCG methylation is presented.
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