ArticleBMC plant biology2015
The lycopene β-cyclase plays a significant role in provitamin A biosynthesis in wheat endosperm.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
- Genome-Wide Identification of LCY Genes inPlants (Basel, Switzerland) · 2026Article
- Analysis of lycopene variation and genetic characteristics in wheat grain.BMC plant biology · 2025Article
- A multi-omic resource of wheat seed tissues for nutrient deposition and improvement for human health.Scientific data · 2023Article
- Genetic and molecular basis of carotenoid metabolism in cereals.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Article
- An established protocol for generating transgenic wheat for wheat functional genomicsFrontiers in plant science · 2022Article
- Biochemical and Immunological implications of Lutein and Zeaxanthin.International journal of molecular sciences · 2021Review
- A single amino acid insertion in LCYB2 deflects carotenoid biosynthesis in red carrot.Plant cell reports · 2021Article
- Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation inPlants (Basel, Switzerland) · 2021Article
- Article
- A Genome-Wide Association Study Reveals a Rich Genetic Architecture of Flour Color-Related Traits in Bread Wheat.Frontiers in plant science · 2018Article
- Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora.Molecules (Basel, Switzerland) · 2017Article
- Carotenoids in Staple Cereals: Metabolism, Regulation, and Genetic Manipulation.Frontiers in plant science · 2016Review
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15 authors at 1 institution in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundLycopene β-cyclase (LCYB) is a key enzyme catalyzing the biosynthesis of β-carotene, the main source of provitamin A. However, there is no documented research about this key cyclase gene's function and relationship with β-carotene content in wheat. Therefore, the objectives of this study were to clone TaLCYB and characterize its function and relationship with β-carotene biosynthesis in wheat grains. We also aimed to obtain more information about the endogenous carotenoid biosynthetic pathway and thus provide experimental support for carotenoid metabolic engineering in wheat.
resultsIn the present study, a lycopene β-cyclase gene, designated TaLCYB, was cloned from the hexaploid wheat cultivar Chinese Spring. The cyclization activity of the encoded protein was demonstrated by heterologous complementation analysis. The TaLCYB gene was expressed differentially in different tissues of wheat. Although TaLCYB had a higher expression level in the later stages of grain development, the β-carotene content still showed a decreasing tendency. The expression of TaLCYB in leaves was dramatically induced by strong light and the β-carotene content variation corresponded with changes of TaLCYB expression. A post-transcriptional gene silencing strategy was used to down-regulate the expression of TaLCYB in transgenic wheat, resulting in a decrease in the content of β-carotene and lutein, accompanied by the accumulation of lycopene to partly compensate for the total carotenoid content. In addition, changes in TaLCYB expression also affected the expression of several endogenous carotenogenic genes to varying degrees.
conclusionOur results suggest that TaLCYB is a genuine lycopene cyclase gene and plays a crucial role in β-carotene biosynthesis in wheat. Our attempt to silence it not only contributes to elucidating the mechanism of carotenoid accumulation in wheat but may also help in breeding wheat varieties with high provitamin A content through RNA interference (RNAi) to block specific carotenogenic genes in the wheat endosperm.
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