Evidence map›Paper›PMID 25943100›Full record

ArticleBMC medical genomics2015

DNA methylation differences in monozygotic twin pairs discordant for schizophrenia identifies psychosis related genes and networks.

Christina A Castellani, Benjamin I Laufer, Melkaye G Melka, Eric J Diehl, Richard L O'Reilly, Shiva M Singh

Open access · goldAbstract readTwin Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC medical genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 2 pooled it
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Christina A CastellaniDepartment of Biology, The University of Western Ontario, N6A 5B7, London, Ontario, Canada. ccastel3@uwo.ca.
Benjamin I LauferDepartment of Biology, The University of Western Ontario, N6A 5B7, London, Ontario, Canada. blaufer@uwo.ca.
Melkaye G MelkaDepartment of Biology, The University of Western Ontario, N6A 5B7, London, Ontario, Canada. mmelka@uwo.ca.
Eric J DiehlDepartment of Biology, The University of Western Ontario, N6A 5B7, London, Ontario, Canada. ediehl@uwo.ca.
Richard L O'ReillyDepartment of Psychiatry, The University of Western Ontario, N6A 5B7, London, Ontario, Canada. roreilly@uwo.ca.
Shiva M SinghDepartment of Biology, The University of Western Ontario, N6A 5B7, London, Ontario, Canada. ssingh@uwo.ca.
Western University · CA

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

backgroundDespite their singular origin, monozygotic twin pairs often display discordance for complex disorders including schizophrenia. It is a common (1%) and often familial disease with a discordance rate of ~50% in monozygotic twins. This high discordance is often explained by the role of yet unknown environmental, random, and epigenetic factors. The involvement of DNA methylation in this disease appears logical, but remains to be established.

methodsWe have used blood DNA from two pairs of monozygotic twins discordant for schizophrenia and their parents in order to assess genome-wide methylation using a NimbleGen Methylation Promoter Microarray.

resultsThe genome-wide results show that differentially methylated regions (DMRs) exist between members representing discordant monozygotic twins. Some DMRs are shared with parent(s) and others appear to be de novo. We found twenty-seven genes affected by DMR changes that were shared in the affected member of two discordant monozygotic pairs from unrelated families. Interestingly, the genes affected by pair specific DMRs share specific networks. Specifically, this study has identified two networks; "cell death and survival" and a "cellular movement and immune cell trafficking". These two networks and the genes affected have been previously implicated in the aetiology of schizophrenia.

conclusionsThe results are compatible with the suggestion that DNA methylation may contribute to the discordance of monozygotic twins for schizophrenia. Also, this may be accomplished by the direct effect of gene specific methylation changes on specific biological networks rather than individual genes. It supports the extensive genetic, epigenetic and phenotypic heterogeneity implicated in schizophrenia.

Indexed as

DNA MethylationGenetic Predisposition to DiseaseAdultCpG IslandsDiseases in TwinsEpigenesis, GeneticFemaleGene Expression ProfilingGene Expression RegulationGenome, HumanHistonesHumansMiddle AgedMultigene FamilyPedigreePhenotypeHistones

Identifiers

PMID25943100
PMCPMC4494167
OpenAlexW1710157178

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.