Evidence map›Paper›PMID 25942484›Full record

ArticleJournal of neurotrauma2015

Moderate Hypothermia Significantly Decreases Hippocampal Cell Death Involving Autophagy Pathway after Moderate Traumatic Brain Injury.

Yichao Jin, Yingying Lin, Jun-feng Feng, Feng Jia, Guo-yi Gao, Ji-yao Jiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neurotrauma, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yichao JinDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University , School of Medicine, Shanghai, China .
Yingying LinDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University , School of Medicine, Shanghai, China .
Jun-feng FengDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University , School of Medicine, Shanghai, China .
Feng JiaDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University , School of Medicine, Shanghai, China .
Guo-yi GaoDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University , School of Medicine, Shanghai, China .
Ji-yao JiangDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University , School of Medicine, Shanghai, China .
Renji Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we evaluated changes in autophagy after post-traumatic brain injury (TBI) followed by moderate hypothermia in rats. Adult male Sprague-Dawley rats were randomly divided into four groups: sham injury with normothermia group (37 °C); sham injury with hypothermia group (32 °C); TBI with normothermia group (TNG; 37 °C); and TBI with hypothermia group (THG; 32 °C). Injury was induced by a fluid percussion TBI device. Moderate hypothermia (32 °C) was achieved by partial immersion in a water bath (0 °C) under general anesthesia for 4 h. All rats were killed at 24 h after fluid percussion TBI. The ipsilateral hippocampus in all rats was analyzed with hematoxylin and eosin staining; terminal deoxynucleoitidyl transferase-mediated nick end labeling staining was used to determine cell death in ipsilateral hippocampus. Immunohistochemistry and western blotting of microtubule-associated protein light chain 3 (LC3), Beclin-1, as well as transmission electron microscopy performed to assess changes in autophagy. At 24 h after TBI, the cell death index was 27.90 ± 2.36% in TNG and 14.90 ± 1.52% in THG. Expression level of LC3 and Beclin-1 were significantly increased after TBI and were further up-regulated after post-TBI hypothermia. Further, ultrastructural observations showed that there was a marked increase of autophagosomes and autolysosomes in ipsilateral hippocampus after post-TBI hypothermia. Our data demonstrated that moderate hypothermia significantly attenuated cell death and increased autophagy in ipsilateral hippocampus after fluid percussion TBI. In conclusion, autophagy pathway may participate in the neuroprotective effect of post-TBI hypothermia.

Indexed as

Hypothermia, InducedAnimalsApoptosis Regulatory ProteinsAutophagyBeclin-1Body TemperatureBrain InjuriesHippocampusMaleMembrane ProteinsMicrotubule-Associated ProteinsRatsRats, Sprague-DawleyApoptosis Regulatory ProteinsBeclin-1BECN1 protein, humanMembrane ProteinsMicrotubule-Associated Proteinsautophagycell deathhippocampusmoderate hypothermiatraumatic brain injury

Identifiers

PMID25942484
PMCPMC4504336
OpenAlexW1485849891

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.