ArticlePloS one2015
P21 deficiency delays regeneration of skeletal muscular tissue.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Cdkn1a silencing restores myoblast differentiation by inducing selective apoptosis in senescent cells.Cellular & molecular biology letters · 2025Article
- Osmolar Modulation Drives Reversible Cell Cycle Exit and Human Pluripotent Cell Differentiation via NF-κВ and WNT Signaling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- High-fat stimulation induces atrial neural remodeling by reducing NO production via the CRIF1/eNOS/P21 axi.Lipids in health and disease · 2023Article
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- Muscle Stem Cell Function Is Impaired in β2-Adrenoceptor Knockout Mice.Stem cell reviews and reports · 2022Article
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- XBP1u Is Involved in C2C12 Myoblast DifferentiationFrontiers in physiology · 2022Article
- Porcine ZBED6 regulates growth of skeletal muscle and internal organs via multiple targets.PLoS genetics · 2021Article
- Susceptibility of cyclin-dependent kinase inhibitor 1-deficient mice to rheumatoid arthritis arising from interleukin-1β-induced inflammation.Scientific reports · 2021Article
- Uncovering the PIDDosome and caspase-2 as regulators of organogenesis and cellular differentiation.Cell death and differentiation · 2020Review
- In vivo analysis of γH2AX+ cells in skeletal muscle from aged and obese humans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Alterations of cell cycle genes in cancer: unmasking the role of cancer stem cells.Molecular biology reports · 2020Review
- Aging Induced p53/p21 in Genioglossus Muscle Stem Cells and Enhanced Upper Airway Injury.Stem cells international · 2020Article
- Article
- The Multifaceted p21 (Cip1/Waf1/Cancers · 2019Review
- Skeletal Muscle Pathophysiology: The Emerging Role of Spermine Oxidase and Spermidine.Medical sciences (Basel, Switzerland) · 2018Review
- p21BMC musculoskeletal disorders · 2017Article
- Transcutaneous carbon dioxide application accelerates muscle injury repair in rat models.International orthopaedics · 2017Article
- CDK inhibitors for muscle stem cell differentiation and self-renewal.The journal of physical fitness and sports medicine · 2017Article
- Myoprotective effects of bFGF on skeletal muscle injury in pressure-related deep tissue injury in rats.Burns & trauma · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potential relationship between cell cycle checkpoint control and tissue regeneration has been indicated. Despite considerable research being focused on the relationship between p21 and myogenesis, p21 function in skeletal muscle regeneration remains unclear. To clarify this, muscle injury model was recreated by intramuscular injection of bupivacaine hydrochloride in the soleus of p21 knockout (KO) mice and wild type (WT) mice. The mice were sacrificed at 3, 14, and 28 days post-operation. The results of hematoxylin-eosin staining and immunofluorescence of muscle membrane indicated that muscle regeneration was delayed in p21 KO mice. Cyclin D1 mRNA expression and both Ki-67 and PCNA immunohistochemistry suggested that p21 deficiency increased cell cycle and muscle cell proliferation. F4/80 immunohistochemistry also suggested the increase of immune response in p21 KO mice. On the other hand, both the mRNA expression and western blot analysis of MyoD, myogenin, and Pax7 indicated that muscular differentiation was delayed in p21KO mice. Considering these results, we confirmed that muscle injury causes an increase in cell proliferation. However, muscle differentiation in p21 KO mice was inhibited due to the low expression of muscular synthesis genes, leading to a delay in the muscular regeneration. Thus, we conclude that p21 plays an important role in the in vivo healing process in muscular injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.