ArticleJournal of experimental botany2015
Accumulation of long-lived mRNAs associated with germination in embryos during seed development of rice.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives.Frontiers in plant science · 2022Pooled it
- Genes, Putative Long-Lived mRNAs and Pathways Underlying Genotypic Differences in Rice Seed Storability and Seed Dormancy.Rice (New York, N.Y.) · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Molecular timekeepers: the curious alliance of redox, repair, and protective proteins in preserving seed longevity.Crop health · 2026Review
- OsHsp20-25, a small heat shock protein, from long-lived mRNA regulates seed size and germination rate in rice.Planta · 2025Article
- A commitment for life: Decades of unraveling the molecular mechanisms behind seed dormancy and germination.The Plant cell · 2024Review
- Insights into mechanisms of seed longevity in soybean: a review.Frontiers in plant science · 2023Review
- Identification of the Potential Genes Regulating Seed Germination Speed in Maize.Plants (Basel, Switzerland) · 2022Article
- Exploring Breakthroughs in Three Traits Belonging to Seed Life.Plants (Basel, Switzerland) · 2022Review
- Article
- Correlation between seed longevity and RNA integrity in the embryos of rice seeds.Plant biotechnology (Tokyo, Japan) · 2021Article
- Barley Seeds miRNome Stability during Long-Term Storage and Aging.International journal of molecular sciences · 2021Article
- Desiccation Tolerance as the Basis of Long-Term Seed Viability.International journal of molecular sciences · 2020Review
- Analysis of Stored mRNA Degradation in Acceleratedly Aged Seeds of Wheat and Canola in Comparison to Arabidopsis.Plants (Basel, Switzerland) · 2020Article
- Low RIN Value for RNA-Seq Library Construction from Long-Term Stored Seeds: A Case Study of Barley Seeds.Genes · 2020Article
- Lost in Translation: Physiological Roles of Stored mRNAs in Seed Germination.Plants (Basel, Switzerland) · 2020Review
- Seed-Stored mRNAs that Are Specifically Associated to Monosomes Are Translationally Regulated during Germination.Plant physiology · 2020Article
- Dissection of soybean populations according to selection signatures based on whole-genome sequences.GigaScience · 2019Article
- Small RNA sequencing reveals dynamic microRNA expression of important nutrient metabolism during development ofInternational journal of biological sciences · 2019Article
- Arabidopsis Seed Stored mRNAs are Degraded Constantly over Aging Time, as Revealed by New Quantification Methods.Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mature dry seeds contain translatable mRNAs called long-lived mRNAs. Early studies have shown that protein synthesis during the initial phase of seed germination occurs from long-lived mRNAs, without de novo transcription. However, the gene expression systems that generate long-lived mRNAs in seeds are not well understood. To examine the accumulation of long-lived mRNAs in developing rice embryos, germination tests using the transcriptional inhibitor actinomycin D (Act D) were performed with the Japonica rice cultivar Nipponbare. Although over 70% of embryos at 10 days after flowering (DAF) germinated in the absence of the inhibitor, germination was remarkably impaired in embryos treated with Act D. In contrast, more than 70% of embryos at 20, 25, 30 and 40 DAF germinated in the presence of Act D. The same results were obtained when another cultivar, Koshihikari, was used, indicating that the long-lived mRNAs required for germination predominantly accumulate in embryos between 10 and 20 DAF during seed development. RNA-Seq identified 529 long-lived mRNA candidates, encoding proteins such as ABA, calcium ion and phospholipid signalling-related proteins, and HSP DNA J, increased from 10 to 20 DAF and were highly abundant in 40 DAF embryos of Nipponbare and Koshihikari. We also revealed that these long-lived mRNA candidates are clearly up-regulated in 10 DAF germinating embryos after imbibition, suggesting that the accumulation of these mRNAs in embryos is indispensable for the induction of germination. The findings presented here may facilitate in overcoming irregular seed germination or producing more vigorous seedlings.
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